| 摘要: |
| 在光镜和电镜下观察了糙海参(Holothuria scabra)精子发生的过程及其形态结构。结果表明,糙海参精子发生经历了精原细胞、初级精母细胞、次级精母细胞、精细胞和成熟精子五个阶段。精细胞分化过程可分为3个时期,早期：细胞核前端出现前顶体囊；中期：顶体腔内出现围顶体物质；后期：胞质往尾部方向收缩,形成精子中部。糙海参精子全长约60μm,由头部、中部和尾部三部分组成,为典型的鞭毛型精子。精子头部呈球形,横轴长约1.8μm,纵轴长约1.4μm,顶体呈“U”型内陷在精子核中,而围顶体物质呈“月牙”形,核染色质凝集成团块状。中部由一个巨大的线粒体和中心粒复合体融合而成。精子尾部细长,鞭毛横切面为典型的“9 2”微管结构。本结果可为糙海参的繁殖生物学和人工育苗提供理论参考。 |
| 关键词: 糙海参 精子发生 形态 超微结构 |
| DOI：10.11759/hykx20141219001 |
| 分类号:Q248 |
| 基金项目:国家自然科学(31260636)；广西自治区直属公益性科研院所项目(GXIF-2014-008) |
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| Spermatogenesis and Sperm Morphology of Sea Cucumber Holothuria scabra |
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YANG Xue-ming1, WU Ming-can2, ZHANG Li1, YANG Qiong1, PAN Ying3, CHEN Fuyan1
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1.Guangxi Academy of Fishery Sciences;2.College of Animal Science and Technology of Guangxi University;3.College of Animal Science and Technology, Guangxi University
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| Abstract: |
| Spermatogenesis and ultrastructure of sperm were observed in sea cucumber Holothuria scabroa by scanning electronic microscopy (SEM), transmission electronic microscopy (TEM) and light microscope.The spermatogenesis was shown to be divided into five stages: spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid and spermatozoon. The differentiation of spermatids was subdivided into three stages, the early spermatid: formation of proacrosomal vesicle at the front of the nucleus; the mid-spermatid: periacrosomal material in the cavity of acrosome; the later spermatid: the cytoplasm shrinking backward and becoming a flagellum, until the formation of central section of the spermatozoon. The mature spermatozoon as a flagellated a type is about 60 μm long, and consists of three parts: head, middle piece and tail. The head is spherical in shape with an apical acrosome which has about 1.8 μm of vertical axis and about 1.4 μm of horizontal length. The acrosome is an U-shaped cystid, and the periacrosomal material space is crescent in shape; the chromatin in the acrosome is condensed into the nucleus. A large mitochondria and centriole complex are in the mid-piece of the spermatozoon, and in the tail, the flagellum is composed of typical “9 2” microtubular structure. The results can be as the theoretical reference for the breeding biology and artificial reproduction technology in sea cucumber Holothuria scabroa. |
| Key words: Holothuria scabra spermatogenesis morphology ultrastructure |