首页 | 期刊介绍 | 编委会 | 道德声明 | 投稿指南 | 常用下载 | 过刊浏览 | In English
引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 177次   下载 0  
分享到: 微信 更多
脂肪营养对循环水养殖道氏虹鳟生长、相关代谢酶及基因表达的影响
张 静1, 李 勇1, 孙国祥1, 赵宁宁1, 马 骏1, 王顺奎2
1.中国科学院海洋研究所;2.山东东方海洋科技股份有限公司
摘要:
为探讨饲料脂肪营养对封闭循环水养殖道氏虹鳟(Oncorhynchus mykiss)生长、脂肪代谢及相关酶基因表达的影响,本试验采用单因素设计,即4个脂肪水平——12%、15%、18%、21%(以Ⅰ、Ⅱ、Ⅲ、Ⅳ组表示),共4个处理组,每处理3重复,每重复25尾鱼。试验鱼为初始体重(333.25±20.71)g的道氏虹鳟幼鱼,试验期77 d。结果表明,1)与后三组相比,Ⅰ组试验鱼增重率显著提高17.20%~33.17%;Ⅰ、Ⅱ组饲料系数显著低于Ⅲ、Ⅳ组6.20%~20.56%(P<0.05)。2)脂肪合成酶类FAS活力随饲料脂肪水平升高而降低,脂肪分解酶类LPL、HL及L-CPT Ⅰ活力变化特征与之相反(P<0.05)。其中,Ⅰ组FAS活力显著高于Ⅲ、Ⅳ组7.5%、8.7%;L-CPT Ⅰ活力显著低于后三组13.08%~16.35%;Ⅳ组LPL活力显著或极显著高于前三组18.8%~44.7%(P<0.05或P<0.01)。3)Ⅰ组肝脏FAS的mRNA表达量比后三组显著高48.29%~55.15%(P<0.05);LPL呈相反特征,Ⅳ组显著高于前三组36.72%~113.59%(P<0.05或P<0.01);Ⅰ组肌肉FAS mRNA表达量显著高于Ⅳ组66.82%(P<0.05);LPL表达丰度随脂肪水平升高先下降后显著上升(P<0.05);该结果特征与其代谢酶活力变化具有同步性。研究发现,肝脏FAS与LPL两种酶基因mRNA表达量随饲料脂肪水平变化的差异性比肌肉更明显。本试验初步确定,在封闭循环水养殖模式下,330 g左右道氏虹鳟饲料蛋白水平在45%时,脂肪水平以不超过12%为宜。
关键词:  脂肪  道氏虹鳟  生长  脂肪代谢  基因表达  循环水养殖
DOI:10.11759/hykx20170303003
分类号:S963.7
基金项目:“十二五”国家科技支撑计划项目(2011BAD13B07);中国科学院院地合作项目(Y12530101L)
Effects of Lipid Levels on Growth, Fatty Enzymatic Activity and Gene Expression of Rainbow Trout (Oncorhynchus mykiss) in recirculating aquaculture system
ZHANG Jing1, LI Yong1, SUN Guoxiang1, ZHAO Ningning1, MA Jun1, WANG Shunkui2
1.Institute of Oceanology,Chinese Academy of Sciences;2.Shandong Oriental Ocean Sci-Tech Co,Ltd
Abstract:
The trial was conducted to evaluate the effects of dietary lipid levels on growth performance, fat metabolism and gene mRNA expression of rainbow trout (Oncorhynchus mykiss) in recirculating aquaculture system (RAS). Four isonitrogenous diets containing 45% crude protein with increasing dietary lipid levels 12%, 15%, 18% and 21%(expressed as group Ⅰ, Ⅱ, Ⅲ, Ⅳ) were fed to satiety to triplicate groups of 25 fishes(333.25±20.71 g)for 77 days. Results are as follows. Firstly, growth performance of group Ⅰ was optimal on account of highest weight gain (77.41%) and lowest feed conversion rate (1.16) (P<0.05 or P<0.01). Secondly, fatty acid synthetase (FAS) activity was decreased with increasing dietary fat content, while the activities of lipolytic enzymes changed in reverse. FAS activity of group Ⅰ was significantly higher than group Ⅲ, Ⅳ by 7.5%, 8.7% respectively, and L-CPT Ⅰ activity of this group was lower than others by 13.08%~16.35%(P<0.05). Meanwhile, LPL activity of group Ⅳ was significantly highest (P<0.05). That meant fishes fed group Ⅰ diet improved fat utilization rate by increasing fat synthesis and decreasing lipolysis, to ensure body normal requirement for lipid and promote growth. Thirdly, FAS mRNA expression in group Ⅰ was higher than others by 48.29%~55.15%(P<0.05), while changes of LPL was on the contrary (P<0.05 or P<0.01). In muscle, FAS mRNA expression in group Ⅰ was higher than group Ⅳ by 66.82% (P<0.05); the expression of LPL was firstly decreased and then increased with increasing dietary lipid levels. Preliminary finding was that FAS and LPL gene expression in liver changed faster than in muscle with the increase of fat levels. Determined primarily, the Donaldsons rainbow trout (around 330 g) could achieve healthy growth without excessive fat supply, and its fat nutrition requirement is no more than 12% in RAS when the dietary protein level is 45%.
Key words:  lipid  Oncorhynchus mykiss  growth  fat metabolism  gene expression  recirculating aquaculture system
版权所有 《海洋科学》 Copyright©2008 All Rights Reserved
主管单位:中国科学院 主办单位:中国科学院海洋研究所
地址:青岛市市南区福山路32号  邮编:266071  电话:0532-82898755  E-mail:marinesciences@qdio.ac.cn
技术支持:北京勤云科技发展有限公司