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蓝身大斑石斑鱼染色体核型分析
刘苏,杨宇晴,张海发,张勇,吴锦辉,黄锦雄,石和荣
1.广东省海洋渔业试验中心;2.广东省海洋渔业试验中心DdDd中山大学水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室
摘要:
为了解蓝身大斑石斑鱼(Epinephelus tukula)的细胞生物学特征,采用体内注射PHA培养肾细胞法对蓝身大斑石斑鱼染色体核型进行了研究。结果表明,其染色体核型为2n=48t,属于石斑鱼属鱼类典型的高位类中原始类群,是石斑鱼属进化过程中出现较早的物种。
关键词:  蓝身大斑石斑鱼  核型  染色体
DOI:
分类号:Q343.22
基金项目:基金项目:公益性行业(农业)科研专项(201403011);广东省海洋和渔业发展专项资金(201743);广东省渔港建设和渔业发展专项资金(201611);广东省海洋渔业科技与产业发展专项(A201400A01,A201600A02);广东省科技计划项目(2015A020208002) [Foundation: Special Fund for Agro-scientific Research in the Public Interest , No.201403011; Special fund for Marine fisheries development in Guangdong province, No.201743; Special fund for fishing port construction and fishery development in Guangdong province, No.201611; Special fund for Marine fishery science and industry development in Guangdong province, No.A201400A01; Science and Technology Planning Project of Guangdong Province, No.2015A020208002]
The karyotype of Epinephelus tukula
liusu1, YANG Yu-qing2, ZHANG Hai-fa1, ZHANG Yong3, WU Jin-hui1, HUANG Jin-xiong1, SHI He-rong1
1.Marine Fisheries Development Center of Guangdong Province;2.Marine Fisheries Development Center of Guangdong ProvinceDdDd Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals;3.Marine Fisheries Development Center of Guangdong ProvinceDdDdInstitute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals
Abstract:
To explore the cell biological characters of Epinephelus tukula,we analyzed the karyotype of E. tukula from their renal tissue by the method of PHA and colchicine injection method. The results showed that there were 48 telocentric chromosomes in the diploid and their karyotypic formula was 2n=48t. It can be concluded E. tukula has the representative characteristics of ancestral karyotypic pattern and belongs to the primitive grouper.
Key words:  Epinephelus tukula  karyotype  chromosome
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