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不同盐度对文蛤呼吸代谢及体内酶活性的影响
聂鸿涛, 白奕天, 丁天扬, 闫喜武
大连海洋大学
摘要:
为研究不同盐度对文蛤呼吸代谢的影响,本实验设置5个盐度梯度(11,18,25,32,39),研究了不同盐度对文蛤 (Meretrix meretrix) 耗氧和排氨的影响以及文蛤的外套膜、鳃、肝胰腺三种组织中乳酸脱氢酶和Na+/K+-ATP 酶活性的变化。结果表明:随着盐度的不断升高,耗氧率先升高后下降,在盐度18时达到最大值;排氨率先升高后下降,在盐度32时达到最大值。随着盐度不断升高和胁迫时间延长,文蛤的肝胰腺中乳酸脱氢酶活力总体呈先升高后下降再升高的趋势(P<0.05) ,酶活力在39盐度时为最高;随着盐度不断升高和胁迫时间延长,文蛤的外套膜中Na+/K+-ATP 酶活力总体呈先下降再升高后下降的趋势(P<0.05),在32盐度时为最高。研究结果为文蛤的人工养殖提供参考。
关键词:  文蛤  盐度  耗氧率  排氨率  乳酸脱氢酶  Na+/K+-ATP 酶
DOI:10.11759/hykx20180207002
分类号:
基金项目:国家科技攻关计划,国家高技术研究发展计划(863计划)
Effects of different salinity on respiratory metabolism and enzyme activities of Meretrix meretrix
NIE Hong-Tao, BaiYitian, DingTianyang, YanXiwu
Engineering and Technology Research Center of Shellfish Breeding of Liaoning Province,Dalian Ocean University
Abstract:
In order to study the effects of salinity on the respiratory metabolism of Meretrix meretrix, five salinity gradients (11, 18, 25, 32, and 39) were set up to investigate the effects of acute salinity change on the oxygen consumption and ammonia excretion of M. Meretrix, and the activity of lactate dehydrogenase (LDH) and Na+/K+-ATPase in the mantle, gills and hepatopancreas of M. Meretrix. The results showed that with the increase of salinity, the oxygen consumption rate was first increased and then decreased, and the maximum value reached at salinity of 18, and the ammonia excretion rate was first increased and then decreased, and the maximum reached at salinity of 32. As the salinity increases and the time of stress is prolonged, the activity of LDH in the hepatopancreas of M. Meretrix was increased firstly, then decreased and then increased (P < 0.05), and the enzyme activity was highest in 39 salinity. With the increase of salinity and the prolongation of stress time, the ATP activity in the mantle of M. Meretrix was decreased firstly, then increased and then decreased (P > 0.05) , which was highest at 32 salinity. The results provided a reference for the artificial culture technique of M. Meretrix.
Key words:  Meretrix meretrix  acute salinity change  oxygen consumption rate  ammonia excretion rate  lactate dehydrogenase (LDH)  Na+/K+-ATPase
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