摘要: |
利用辽宁大连的紫贻贝与福建宁德的厚壳贻贝为亲本,采用2×2双列杂交设计,构建了紫贻贝自交组(M.Galloprovincialis♀×M.Galloprovincialis♂)、正交组(M.Galloprovincialis♀×M. Coruscus♂)、反交组(M. Coruscus♀×M.Galloprovincialis♂)与厚壳贻贝自交组(M. Coruscus♀×M. Coruscus♂)4个遗传组合,比较分析了受精率、孵化率、子代的成活率、生长数据等,探讨了紫贻贝与厚壳贻贝种间杂交的可行性,同时为贻贝良种选育提供参考。结果表明,自交组与反交组的受精强度具有不对称性,正交组与自交组受精率无差异,反交组的受精率及孵化率显著低于正交组与自交组(p<0.05),且浮游幼虫不能正常存活;正交组子代具有生长优势,第9、11、13天的浮游幼虫及第78天的稚贝壳长均显著大于自交组(p<0.05);正交组与紫贻贝自交组在整个幼虫阶段存活率无显著差异,在壳顶幼虫期显著高于厚壳贻贝自交组(p<0.05)。 |
关键词: 紫贻贝、厚壳贻贝、种间杂交、生长、存活 |
DOI: |
分类号: |
基金项目:现代农业产业技术体系建设专项(CARS-49) |
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Comparison of growth and survival among the hybrid offspring of Mytilus galloprovincialis and M. coruscus from laboratory crossesYang Linying1,2,3, Liu Mingkun1,3, Que Huayong1,3*, Zhang Guofan1,3 |
yanglinying,liumingkun,quehuayong,zhangguofan
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Institute of Oceanology, Chinese Academy of Sciences
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Abstract: |
In order to clarify the feasibility of interspecific hybridization between the two commercially important mussel species of M. galloprovincialis and M. coruscus, a diallel cross was conducted with 3 replicates using the brood-stock of M. galloprovincialis from Dalian of Liaoning and M. coruscus from Ningde of Fujian, respectively. Two cross groups, i.e., M. coruscus♀×M. galloprovincialis♂ and M. coruscus♀×M. galloprovincialis were established, along with the two purebred groups (M. galloprovincialis♀×M. galloprovincialis♂, M. coruscus♀×M. coruscus♂) as controls. The development and growth were compared by monitoring of fertilization, hatching, survival and growth of the offspring from the four groups. Results showed that the fertilization rate exhibited notably asymmetric between the two cross groups. Mean fertilization rate of 88.87% was observed in the cross-group of M. galloprovincialis♀×M. coruscus♂, which was not significantly different from the two purebred groups. The fertilization and hatching rate of cross-group M. coruscus♀×M. galloprovincialis♂ were significantly lower than all the other groups(p<0.05), resulting in no offspring obtained eventually. The offspring from cross-group M. galloprovincialis♀×M. coruscus♂ showed notable advantage in growth, as evidenced by the shell length of the planktonic larvae on day 9, 11, 13 and the juvenile on day 78 were significantly larger than the purebred groups(p<0.05). The survival rate of cross-group M. galloprovincialis♀×M. coruscus♂ had no significant difference from the purebred group M. galloprovincialis♀×M. galloprovincialis♂ during the planktonic larvae stage, but significantly higher than the purebred group M. coruscus♀×M. coruscus♂(p<0.05). The results of this study provided a valuable information for mussels breeding program in the future. |
Key words: Mytilus galloprovincialis M. coruscus interspecific hybridization growth survival |