| 摘要: |
| 为了研究海洋生物污损对船体和海洋工程的影响，本文将热喷涂镀钴基、镍基及含铜氧化钛基膜的船用厚钢板试样片放置在海水中，利用振荡培养箱模拟海洋环境，进行晃片培养。分离纯化镀膜钢片表面上的细菌，研究早期污损细菌的生长规律，通过革兰氏染色、生理生化实验对细菌进行鉴定，伯杰氏系统细菌学手册查证。采用纸片法测定抑菌圈，研究铜、钴、镍三种金属离子对污损细菌的影响，观察不同离子浓度的抑菌圈。研究结果表明：早期海洋污损细菌有G+芽孢杆菌属、G+芽孢乳杆菌属、G+无芽孢杆菌、葡萄球菌属、G+无芽孢丝状菌、假单胞菌属六种属；其中G+细菌首先吸附；Cu2+的最佳抑制浓度为30g/l，Co2+的最佳抑制浓度为80g/l，Ni2+的最佳抑制浓度为110g/l。
关键词：海洋污损；早期污损细菌；金属离子；抑菌作用 |
| 关键词: 海洋污损 早期污损细菌 金属离子 抑菌作用 |
| DOI： |
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| Separation and Purification of Marine Fouling Bacteria and Inhibition of Metal Ions |
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xiasuhang, quanyanling
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University of Science and Technology Liaoning
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| Abstract: |
| Marine biofouling impacts dramatically on hull and ocean engineering. The hot-sprayed cobalt-based, nickel-based and copper-titanium-containing base film-coated marine thick steel plate samples were placed in sea water. The sea environment was simulated by a shaking incubator . The bacteria on the surface of the coated steel sheet were separated and purified, and the growth law of the early fouling bacteria was studied. The bacteria were identified by Gram staining, physiological and biochemical experiments. Also they were checked by the Berger's system bacteriology manual. The inhibition zone was determined by the paper method, and the effects of three metal ions of copper, cobalt and nickel on one strain of bacteria were studied, and the inhibition zone of different ion concentrations was observed. The results showed that at the early marine fouling stage, the bacteria included G+ Bacillus, G+ bacillus, G+ no Bacillus, Staphylococcus, G+ no spore filamentous fungi, and Pseudomonas; G+ bacteria were adsorpted first. the optimal inhibitory concentration of Cu2+ was 30g/l, Co2+ was 80g/l, and Ni2+ was 110g/l, respectively. |
| Key words: Marine fouling Early fouling bacteria Metal ions Bacteriostasis |