首页 | 期刊介绍 | 编委会 | 道德声明 | 投稿指南 | 常用下载 | 过刊浏览 | In English
引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 159次   下载 0  
分享到: 微信 更多
浒苔发酵工艺优化及青蛤喂养效果评价
夏青1, 孙泽鹏1, 柳梅梅1, 廖晓婷1, 郑汉丰2, 董志国1
1.江苏海洋大学;2.中国水产研究所
摘要:
本试验以自然海区打捞上岸的浒苔(Enteromorpha prolifera)为原料,以植物乳酸杆菌为发酵菌种,发酵浒苔喂养青蛤的特定生长率为响应值,确定浒苔发酵的最佳工艺。在单因素试验的基础上,以发酵时间、发酵温度、料液比为响应因素,以青蛤特定生长率为响应值,利用Box-Behnken中心组合方法进行三因素三水平试验设计,进行响应面分析。结果表明:影响浒苔发酵工艺的因素主次顺序为发酵温度、料液比和发酵时间,最佳发酵工艺条件为:发酵温度35℃,料液比1:1.5,发酵时间84 h。通过测定发酵前后浒苔的常规营养成分,发现浒苔发酵产物的粗蛋白含量显著高于未发酵浒苔(P<0.05);使用工艺优化后的浒苔发酵产物所喂养的青蛤与投喂小球藻的青蛤在特定生长率与存活率方面无显著差异。本试验证明优化后的浒苔发酵工艺具有可靠性,其发酵产物具备成为青蛤养殖饲料的条件,为浒苔发酵饲料的开发利用提供理论依据的同时,也为贝类配合饲料的开发及应用提供极大参考价值。
关键词:  浒苔  青蛤  发酵工艺  响应面法  工艺优化
DOI:
分类号:
基金项目:财政部和农业农村部:国家现代农业产业技术体系资助;江苏省优势学科建设工程资助项目;江苏省高等学校自然科学研究重大项目(18KJA240001);江苏省渔业科技类项目(Y2018-27);国家重点研发计划“蓝色粮仓科技创新”重点专项(2019YFD0900403-08);江苏省研究生科研与实践创新计划项目(No. SJCX20_1274)
Optimization of Enteromorpha prolifera fermentation technology and evaluation of Cyclina sinensis feeding effects
XIA QING1, SUN ZENGPENG1, LIU MEIMEI1, LIAO XIAOTING1, ZHENG HANFENG2, DONG ZHIGUO1
1.Jiangsu Ocean University;2.East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
Abstract:
In this study, Lactobacillus plantarum was used for fermentation strains and the solid-state fermentation method was adopted for microbial fermentation of Enteromorpha prolifera. According to the single factor experiment, we explored the influence of three factors (fermentation time, fermentation temperature and material-liquid ratio) on the fermentation of E. prolifera. The optimal conditions for fermentation of E. prolifera were obtained by orthogonal test. Then we used response surface method to optimize E. prolifera fermentation process. In addition, the study also carried out a culture experiment of clam Cyclina sinensis. By comparing the growth performance of Chlorella and fermented E. prolifera on feeding C. sinensis to verify the actual effect of the fermentation process. The result shows that: (1) The primary and secondary order of the three factors affecting the fermentation process of E. prolifera is fermentation temperature, material-to-liquid ratios and fermentation time. E. prolifera fermented conditions were as follows: fermentation temperature was 35 ℃, material-to-liquid ratios was 1:1.5, and fermentation time was 84 h. (2) It was found that the crude protein content of fermented E. prolifera were significantly higher than it of unfermented E. prolifera (P<0.05). (3) There was no significant difference in specific growth and survival rate of C. sinensis between feeding with Chlorella and fermented E. prolifera. The experimental results provide a theoretical basis for the application of E. prolifera feedstuff in actual production.
Key words:  Enteromorpha prolifera  Cyclina sinensis  fermentation process  response surface methodology  process optimization
版权所有 《海洋科学》 Copyright©2008 All Rights Reserved
主管单位:中国科学院 主办单位:中国科学院海洋研究所
地址:青岛市市南区福山路32号  邮编:266071  电话:0532-82898755  E-mail:marinesciences@qdio.ac.cn
技术支持:北京勤云科技发展有限公司