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广西北部湾钝缀锦蛤精巢发育、精子发生及超微结构研究
连昌朋1, 王超奇1, 杨凌1, 吴韬2, 李胜永2, 潘英2
1.广西大学 动物科学技术学院;2.广西大学
摘要:
2020年11月至2021年10月,本文采用组织切片、扫描电镜、透射电镜技术对广西北部湾海域钝缀锦蛤(Tapes conspersus)的精巢发育过程、生殖细胞的发育、精子的超微结构进行了研究。研究结果显示:目前广西北部湾钝缀锦蛤未见雌雄同体情况,观察的个体皆为雌雄异体。一周年的采样结果中,雌性个体147个,雄性个体93个,雌雄的性比约为1.4:1。1年即为精巢发育的一个周期,发育时期可以划分为增殖期、生长期、成熟期、排放期、休止期共5个主要分期。精细胞的发育可以划分为5个主要分期,即精原细胞期、初级精母细胞期、次级精母细胞期、精细胞期、成熟精子期。精子全长约59.27 μm,属于鞭毛型,由头部、中部、尾部三个部分共同构成,精子头部呈圆锥型,由细胞核和顶体组成,顶体呈倒“V”型。电子在顶体的分布是不均匀的,顶体前部电子分布少,后部分布多。中部主要由两部分构成,即5个圆形排列的线粒体和一个中心粒复合体。轴丝与质膜两个部分共同构成了尾部,轴丝的双联体微管结构横切面表现为“9+2”型,部分精子尾部可以观察到双尾结构。
关键词:  钝缀锦蛤  精巢发育  精子发生  超微结构
DOI:
分类号:S968.31
基金项目:广西创新驱动发展专项(桂科AA19254032); 国家重点研发计划项目(2018YFD0901400)
Studies on the testis、germ cell development and sperm ultrastructure of Tapes conspersus inhabiting Beibu Gulf, Guangxi, China
LIAN CHANG PENG,WANG CHAOQI,YANG LING,WUTAO,LI SHENGYONG,PAN YING
Guangxi University
Abstract:
Abstract: To understand the annual testis development, spermatogenesis and ultrastructure of mature sperm of Tapes conspersus in the Beibu Gulf of Guangxi. In this experiment, the development of testis, spermatogenesis and ultrastructure of T. conspersus were studied by tissue section, scanning electron microscope and transmission electron microscope. Research shows: At present, there is no hermaphrodite in the Tapes conspersus in Beibu Bay of Guangxi, and all the observed individuals are dioecious. The first anniversary of the sampling results, There are 138 females and 102 males. The sex ratio between male and female is about 1.35:1, 1 year is a cycle of testis development, The developmental period can be divided into five main stages: the proliferation period, the growth period, the mature period, the discharge period, and the resting period. The development of sperm cells can be divided into five main stages, namely, spermatogonia, primary spermatocytes, secondary spermatocytes, spermatocytes, and mature spermatids. The total length of the sperm is about 59.27 μm, which is of flagella type. It is composed of three parts: head, middle and tail. The head of the sperm is conical, composed of nucleus and acrosome, and the acrosome is inverted “V” shape. The distribution of electrons in the acrosome is uneven, the distribution of electrons in the front part of the acrosome is less, and the distribution in the back part is more. The middle part is mainly composed of two parts, namely 5 circularly arranged mitochondria and a centriole complex. The two parts of the axon filament and the plasma membrane together constitute the tail. The cross-section of the doublet microtubule structure of the axon is shown as a “9+2” type. In addition, a small number of sperm tails were observed to have a double-tailed structure in this experiment.
Key words:  Tapes conspersus  testis development  Spermatogenesis  ultra-structure
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