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绿鳍马面鲀微卫星标记开发及在丝背细鳞鲀中的通用性检测
王九龙, 徐文刚, 杨沛, 杨婕, 杜荣斌, 刘立明
烟台大学海洋学院
摘要:
绿鳍马面鲀(Thamnaconus modestus)曾是我国重要的海洋捕捞鱼类之一,但其野生资源现已大幅减少。利用微卫星分子标记对绿鳍马面鲀开展群体遗传学研究可为制定科学合理的资源保护和管理措施提供理论依据。本研究利用NCBI数据库中公布的绿鳍马面鲀全基因组序列筛查微卫星位点并在野生群体中验证,在85个选取的位点中筛选得到30个新的多态性较高的微卫星标记。每个微卫星标记的等位基因数为4~16,平均为8.5个;观测杂合度范围为0.207~0.916,平均值为0.685;期望杂合度范围为0.315~0.971,平均值为0.756;多态信息含量范围为0.301~0.898,平均值为0.716,其中属于高度多态的位点有26个,占总位点数的86.67%;经过Bonferroni校正后,有7个位点显著偏离Hardy-Weinberg平衡。将30个绿鳍马面鲀多态性微卫星标记在丝背细鳞鲀(Stephanolepis cirrhifer)中进行通用性检测,其中13个位点成功扩增,9个位点具有多态性。本研究开发的微卫星标记可用于绿鳍马面鲀及相关物种的遗传多样性分析、QTL定位以及系统进化等研究。
关键词:  绿鳍马面鲀  丝背细鳞鲀  微卫星标记  通用性
DOI:
分类号:S917.4
基金项目:
Development of microsatellite markers for Thamnaconus modestus and their transferability in Stephanolepis cirrhifer
WANG Jiu-long, XU Wen-gang, YANG Pei, YANG Jie, Du Rong-bin, LIU Li-ming
College of Ocean,Yantai University
Abstract:
Thamnaconus modestus was one of the most important marine capture target species in China, but its wild resource has been reduced greatly. Studies on population genetics of T. modestus by using microsatellite markers would provide theoretical basis for making scientific resource conservation and management policy. In the present study, microsatellite loci were screened in the complete genome sequence of T. modestus published in NCBI database and verified in a wild population. Thirty novel microsatellite markers with high polymorphism were identified in 85 selected loci. The number of alleles of microsatellite markers ranged from 4 to 16, with an average of 8.5. The observed heterozygosity ranged from 0.207 to 0.916, with an average of 0.685. The expected heterozygosity ranged from 0.315 to 0.971, with an average of 0.756. The content of polymorphic information ranged from 0.301 to 0.898, with an average of 0.716. Of the 30 microsatellite loci, 26 (86.67%) were highly polymorphic and 7 were deviated from Hardy-Weinberg equilibrium significantly after Bonferroni correction. The transferability of these polymorphic microsatellites markers was assessed in the closely-related species Stephanolepis cirrhifer, 13 loci were successfully amplified and 9 loci were polymorphic. The microsatellite markers developed in this study can be used in genetic diversity analysis, QTL mapping and phylogeny study for T. modestus and related species.
Key words:  Thamnaconus modestus  Stephanolepis cirrhifer  microsatellite markers  transferability
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