| 摘要: |
| 为研究不同环境因素对紫贻贝（Mytilus galloprovincialis）幼贝足丝分泌的影响，选取山东省日照市养殖区的紫贻贝幼贝为研究对象，通过区组设计分别进行不同温度（17℃、22℃、27℃）和盐度（20‰、25‰、30‰），不同光照（10lx、100lx、10000lx）和饥饿（0h、24h、48h），不同干露（0h、24h、48h）和氨氮（0、15mg/L、30mg/L）条件的2因素交互作用实验。结果显示，在实验温、盐条件下温度和盐度均会对紫贻贝幼贝足丝分泌产生极显著影响(P<0.01)，均呈显著弱负相关（r=-0.11/-0.16， P<0.05），其中温度27℃、盐度30%组别在不同时间点的紫贻贝幼贝足丝分泌能力显著低于其他组别(P<0.05)。光照对紫贻贝幼贝足丝分泌产生极显著影响(P<0.01)，呈现极显著弱相关（r=0.14，P<0.01），而短期饥饿对紫贻贝幼贝足丝分泌影响不显著(P>0.05)，低光照组（10 lx）紫贻贝幼贝足丝分泌能力显著高于其他组别。干露和氨氮均会对紫贻贝幼贝足丝分泌产生显著影响(P<0.05)，其中干露与紫贻贝足丝分泌显著弱负相关（r=-0.09，P<0.05），而氨氮则显著负相关（r =-0.45，P<0.05），高氨氮（30mg/L）和干露（48h）组合紫贻贝幼贝足丝分泌能力显著低于其他组别(P<0.05)。研究结果可为紫贻贝的苗种转运、夹苗和健康养殖等生产实践提供有益参考。 |
| 关键词: 紫贻贝 足丝分泌 环境因素 相关性分析 |
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| 基金项目:山东省农业良种工程项目“鲍、扇贝和牡蛎等贝类种业创新能力提升”（2024LZGCQY003）；山东省农业良种工程项目“水产种质资源挖掘与精准鉴定”（2021LZGC027）；山东省农业良种工程项目“海水贝类新品种培育”（2022LZGC015） |
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| The Effects of Several Environmental Factors on Byssus Production in Mytilus galloprovincialis |
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zhang shoudu1, Zhang Wei1, Wu Yingying1, Zhen Xiuwei2, Zou Jie3, Song Aihuan1
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1.Marine Science Research Institute of Shandong Province（National Oceanographic Center ,Qingdao）;2.Tianjin Agricultural University;3.Guangxi Academy of Marine Sciences
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| Abstract: |
| To study the effects of environmental factors such as temperature, salinity, light, starvation, exposure in air and ammonia-nitrogen on the production of byssus in Mytilus galloprovincialis, the juvenile blue mussels from the cultivation raft of Rizhao, Shandong Province were selected as the research objects. Two factor interaction experiments with different temperature (17 ℃, 22 ℃, 27 ℃) and salinity (20‰, 25‰, 30‰), different light (10lx, 100lx, 10000lx) and starvation (0h, 24h, 48h), different air exposure (0h, 24h, 48h) and ammonia-nitrogen (0, 15mg/L, 30mg/L) were conducted by block design. The results showed that both temperature and salinity used above had a significant impact on the production of byssus in juvenile blue mussels (P<0.01). The byssus production of juvenile blue mussels in the 27 ℃and 30% salinity group was significantly lower than that in other groups at all experiment time points (P<0.05). Within the experimental range, temperature and salinity showed a significant weak negative correlation (r=-0.11/-0.16, P<0.05) with the production of byssus in juvenile blue mussels. Light has a highly significant effect on the production of byssus in juvenile blue mussels (P<0.01), while short-term starvation has no significant effect on that(P>0.05). The dark group (10lx) has a significantly higher production of byssus than other groups, and there is a weak correlation between light and byssus production in juvenile blue mussels (r=0.14, P<0.01). Both air exposure and ammonia-nitrogen have a significant effect on the production of byssus in juvenile blue mussels (P<0.05). The high ammonia-nitrogen (30mg/L) and air exposure (48h) significantly constrict the production of byssus in juvenile blue mussels (P<0.05). Air exposure is significantly weakly negatively correlated (r=-0.09, P<0.05)with the production of byssus in juvenile blue mussels, while ammonia-nitrogen is significantly negatively correlated (r=-0.45, P<0.05) with the production of byssus in juvenile blue mussels. The research results can provide useful references for the practice of seedling transportation, clamping, and healthy cultivation of blue mussels. |
| Key words: Mytilus galloprovincialis Byssus production Environmental factors Correlation analysis |