摘要: |
经过含H2O2的平板初筛和摇瓶发酵复筛,从保存的水样中筛选到一株过氧化氢酶高产菌株CE-1,其所产过氧化氢酶活性和比活分别为1356.2U/mL和3401U/mg。菌株CE-1为革兰氏阴性杆状细菌,根据其形态特征、16S rRNA基因序列系统进化树分析和生理生化特性以及属内不同种间性状差异等因素的综合分析,将菌株CE-1鉴定为气单胞属(Aeromonas)细菌。 |
关键词: 过氧化氢酶,分离,鉴定,系统发育学分析 |
DOI: |
分类号:Q554+.6 |
基金项目:国家高技术研究发展(863)计划(2007AA091602)和中国水产科学研究院黄海水产研究所基本科研业务费专项资金 |
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Isolation and identification of catalase-producing strain CE-1 |
ZHANG Zeng-xiang,WANG Wei,NIU Rui,SUN Mi
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1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences 2.Shanghai Ocean University;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
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Abstract: |
Catalase-producing strain CE-1 was isolated from water samples by using plate containing H2O2 screening and activity assay of shake flask culture method. The value of the activity and specific activity was 1356.2U/mL and 3401U/mg respectively. CE-1 was Gram negative and rod in shape. According to the comprehensive analysis of morphological and physiological characteristics, the 16S rDNA phylogenetic tree and the differences among different species of Aeromonas, the identification indicated that the strain CE-1 belonged to Aeromonas. |
Key words: Catalase,Isolatation,Identification,Phylogenetic analysis |