| 摘要: |
| 蓝光是海水中能传播的主要光谱，其对海带的生长和发育起重要作用。隐花色素/光裂解酶家族（Cryptochrome/Photolyase Family, CPF）是一类广泛存在的能被蓝光诱导激活的蛋白家族。CRY-DASH (Cryptochrome-Drosophila, Arabidopsis, Synechocystis, Human)是该家族的新成员。本研究利用cDNA末端快速克隆（RACE）技术，获得海带CRY-DASH基因（SjCRY-DASH）全长序列，结构分析发现，其ORF区长1779 bp，编码592个氨基酸。进行氨基酸同源序列比对，其与其它藻类和高等植物间存在两个重要辅基MTHF和FAD结合的保守域。通过不同光质照射诱导海带幼孢子体，我们发现蓝光、白光诱导1 h后，均能使SjCRY-DASH转录水平上升，且SjCRY-DASH对蓝光的响应更强烈。本研究结果为研究大型褐藻-海带中CRY-DASH受光诱导调控功能打下基础。 |
| 关键词: 蓝光 海带 CRY-DASH 孢子体 |
| DOI：10.11759/hykx20170222003 |
| 分类号:X55 |
| 基金项目:国家海洋公益性行业科研专项（201405040）；青岛海洋科学与技术国家实验室计划项目（2015ASKJ02） |
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| Cloning and transcriptional analysis of CRY-DASH from Saccharina japonica (Laminariales, Phaeophyceae) |
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Li Lu
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Institute of Oceanology, Chinese Academy of Sciences
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| Abstract: |
| Blue light is predominant below seawater surface, and it plays a critical role in regulating growth and development of Saccharina japonica. The cryptochrome/photolyase family (CPF) is a diversified protein family which can be induced and activated by blue light. CRY-DASH (Cryptochrome-Drosophila, Arabidopsis, Synechocystis, Human) is a new member of this family. Here we characterize the full-length cDNA of CRY-DASH from S. japonica (designated as SjCRY-DASH) using rapid-amplication of cDNA ends (RACE). The open reading frame (ORF) length of SjCRY-DASH is 1779 bp, encoding 592 amino acids. By multiple alignments of protein sequences, conserved residues that interact with two crucial cofactors, MTHF and FAD, are detected between SjCRY-DASH and several DASH-type proteins of other algae and higher plant. Juvenile sporophytes are induced by light of different wavelengths. Both blue light and white light upregulate the transcriptional level of SjCRY-DASH in 1 h, though stronger response to blue light was found for SjCRY-DASH than white light. Our results lay foundation for future study of light induction and regulation on CRY-DASH of S. japonica. |
| Key words: blue light Saccharina japonica CRY-DASH sporophytes |