摘要: |
以大黄鱼(Larimichthys crocea)为研究对象,通过探究急性低盐度胁迫对大黄鱼血清生化组分的影响,评估盐度骤降引起鱼体的应激反应。急性低盐度(15‰、8‰)胁迫下,在72 h的试验周期中,大黄鱼血清葡萄糖(GLU)含量整体显著下降(P < 0.05);血清总蛋白(TP)、白蛋白(ALB)及球蛋白(GLB)含量在15‰盐度胁迫下呈波动变化,与对照组相比无显著性差异(P > 0.05),8‰盐度胁迫下,TP含量呈显著下降后逐渐升高趋势;血清甘油三酯(TG)和总胆固醇(CHOL)含量在15‰盐度胁迫下均在初始阶段显著变化后逐渐恢复至对照组水平,而8‰低盐胁迫后二者含量均在试验后期(48 h和72 h)大幅度显著升高;血清中的丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)含量在低盐胁迫下均显著高于对照组水平,且呈现出盐度相关性。研究结果表明,急性低盐胁迫会引起鱼体应激反应,实际生产中应避免养殖环境盐度的剧烈变化。 |
关键词: 大黄鱼 急性低盐胁迫 应激反应 血清生化组分 |
DOI: |
分类号:S965.322 |
基金项目: |
|
Effects of acute low salinity stress on serum biochemical components in Larimichthys crocea |
HE Liang-yin1, LI Jin-shou2, SHI Xiao-li2, ZHOU Feng-fang2, HUANG Wei-qing2
|
1.Ningde Normal University;2.College of Life Science,Ningde Normal University
|
Abstract: |
The serum biochemical components were investigated to evaluate the stress response of Larimichthys crocea under acute low salinity shock. The serum biochemical components of L. crocea were measured under 15‰ and 8‰ salinity stress at different timepoint (3 h, 6 h, 12 h, 24 h, 48 h, 72 h) compared with control group. The glucose (GLU) contents in serum of L. crocea decreased significantly in group 15‰ and group 8‰ within 72 h (P < 0.05) compared to group 23‰. The total protein (TP), Albumin (ALB) and globulin (GLB) content in group 15‰ fluctuated during 72 h,showed no significant differences with control, while TP contents dropped significantly in 3 h (P < 0.05) and increased gradually under 8‰ salinity stress. The triglyceride (TG) and cholesterol (CHOL) contents changed significantly in the initial stage, then returned to the control level gradually in group 15‰, however, the contents of two components boosted significantly in the later period under 8‰ salinity stress. The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) contents were significantly higher than the control group under low salinity stress, and the salinity correlation was observed in both components. The results suggested that acute low salinity stress will cause the stress response of large yellow croaker, drastic changes in the salinity should be avoided in farming. |
Key words: Larimichthys crocea acute low salinity stress stress response serum biochemical components |