| 摘要: |
| 为缩短传统体外培养派琴虫较为漫长的生长周期(20 d),以北海派琴虫(Perkinsus. beihaiensis)为培养对象,探讨了温度、盐度、震荡条件这3种物理因素单独或协同变化下对北海派琴虫的生活史和培养速率的影响。结果显示,在北海派琴虫经过休眠孢子阶段之后,出现一个前游动孢子囊阶段,该阶段以出芽增殖的方式繁殖,繁殖速度十分迅速,但细胞体积较小。体外培养过程中,单一的温度、盐度条件变化对北海派琴虫的培养速率无显著影响；但是,当多个培养条件协同变化下,即温度由35℃降至28℃、盐度35ppt降至25ppt时,北海派琴虫8 d即能完成一个增殖循环,与传统培养方法所需的20 d培养周期相比,大大缩减了培养时间,提高了培养速率；当添加震荡条件时,北海派琴虫12 d即完成增殖,扩增时间明显缩短、培养速率明显提高。 |
| 关键词: 北海派琴虫 前游动孢子囊 盐度 温度 震荡 协同变化 |
| DOI： |
| 分类号:S944 |
| 基金项目:惠州学院教授、博士启动项目(2020JB065)国家现代农业产业技术体系建设专项资金(CARS-49)。作者简介王兆瑞,男,1993年9月出生,硕士研究生,研究方向为贝类病害,联系电话15628446520邮箱1013018533@qq.com；通讯作者王江勇,男,1971年9月生,研究员,从事贝类病害研究,电话13316219660,邮箱wjy104@163.com 几种物理因素对北海派琴虫体外培养的影响 |
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| The influence of several physical factors on the in vitro culture of Perkinsus. beihaiensis |
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Wang Zhaorui1, Yang xiaotong2, Wang jiangyong1, Sun jingfeng3
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1.Huizhou university;2.Tianjin Agricultural University,tianjin;3.Tianjin Agricultural University
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| Abstract: |
| Perkinsus beihaiensis was first discovered as a new Perkinsus sp. in the North Sea of China, and it is widespread in China"s coastal waters. Perkinsosis is widespread in the world, and responsible for mass mortality of various kinds of shellfish. The research group established a complete in vitro ARFTM culture method of Perkinsus sp. in the early stage, on this basis, specifically studied the complete life history of P. beihaiensis in vitro culture and its morphological changes and cell contents in different life histories Variety. And for the in vitro culture of P. beihaiensis, design temperature changes, salinity changes, and the addition of shock conditions to explore the physical factors P. beihaiensis reproduction effect. It laid the foundation for the future research of P. beihaiensis insects, especially in vitro culture. |
| Key words: Perkinsus. Beihaiensis pre-trophozoites salinity temperature shock synergistic changes |