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高盐胁迫对缢蛏幼贝存活和三种酶活性的影响
曹伟1,2, 迟长凤1, 董迎辉3, 毕斯琦4, 刘志鸿2,5, 孙秀俊2,5, 周丽青2,5, 吴彪2,5
1.浙江海洋大学 国家海洋设施养殖工程技术研究中心;2.中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室;3.浙江万里学院 浙江省水产种质资源高效利用技术研究重点实验室;4.天津农学院 天津市水产生态及养殖重点实验室;5.青岛海洋科学与技术试点国家实验室 海洋渔业科学与食物产出过程功能实验室
摘要:
为探讨高盐胁迫对壳长约2 cm缢蛏(Sinonovacula constricta)幼贝生长存活和酶活性的影响,该研究设置盐度分别为20‰(S20)、25‰(S25)、30‰(S30)、35‰(S35)、40‰(S40)和45‰(S45)6个组别,测定各组幼贝死亡率,并计算不同胁迫时间的半致死盐度(LC50);同时研究了S20、S30和S40组幼贝软体部Na+-K+-ATP酶(NKA)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)三种酶在不同胁迫时间的活性变化特征。结果表明,实验处理120 h后,各组死亡率分别为0 %、2.17±0.85 %、9.50±0.82 %、30.67±3.70 %、73.50±7.08 %和94.67±3.06 %,168 h时S45组的缢蛏幼贝全部死亡;直线内插法计算得到的72 h、96 h、120 h、144 h、168 h的高盐LC50分别为46.03‰、39.85‰、35.77‰、34.57‰、33.69‰,概率单位法得到的高盐LC50分别为44.31‰、40.74‰、36.96‰、35.67‰、34.47‰。随着盐度升高和处理时间延长,NKA、AKP与ACP活性总体上呈现先上升再下降后稳定的趋势,三种酶活性变化趋势相似,但效应时间不同,盐度高于30‰会给幼贝机体造成较大伤害,能降低机体渗透压调节和免疫防御机能,甚至导致死亡。研究结果为进行缢蛏科学养殖提供了参考数据。
关键词:  缢蛏幼贝  高盐胁迫  半致死盐度  酶活性
DOI:
分类号:S917.4
基金项目:宁波市科技创新2025重大专项(2019B10005);国家海洋水产种质资源库项目
Effects of high salt stress on survival and enzyme activities of juvenile of Sinonovacula constricta
CAO Wei1,2, CHI Chang-feng1, DONG Ying-hui3, BI Si-qi4, LIU Zhi-hong2,5, SUN Xiu-jun2,5, ZHOU Li-qing2,5, WU Biao2,5
1.Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs;3.Zhejiang Wanli University, Key Laboratory of Aquatic Germplasm Resources of Zhejiang;4.Tianjin Agricultural College, Tianjin Key Laboratory of Aqua-ecology and Aquaculture College of Fisheries;5.Qingdao National Laboratory for Marine Science and Technology, Laboratory for Marine Fisheries Science and Food Production Processes
Abstract:
In order to investigate the effects of high salt stress on the survival and enzyme activity of Sinonovacula constricta juvenile with shell length of about 2 cm, the individuals were randomly divided into six groups with different salinities of 20‰ (S20), 25‰ (S25), 30‰ (S30), 35‰ (S35), 40‰ (S40) and 45‰ (S45).The mortalities of S. constricta juvenile in each group were calculated, and the half lethal salinity (LC50) at different stress times were calculated. And also, the activities of Na+ - K+ - ATPase (NKA), acid phosphatase (ACP) and alkaline phosphatase (AKP) of the individuals from groups S20, S30 and S40 were analyzed. The results showed that the mortality rates of groups S20, S25, S30, S35, S40 and S45 at 120 h were 0 %、2.17±0.85 %、9.50±0.82 %、30.67±3.70 %、73.50±7.08 % and 94.67±3.06 %, respectively, and all the individuals of group S45 died at 168 h. The LC50 of high salinity at 72 h, 96 h, 120 h, 144 h and 168h analyze using linear regression method were 46.03‰、39.85‰、35.77‰、34.57‰ and 33.69‰ respectively, and the that obtained by probit analysis method were 44.31‰, 40.74‰, 36.96‰, 35.67‰ and 34.47‰ respectively. With the increase of salinity and the extension of treatment time, the activities of NKA, AKP and ACP first increased, and then decreased followed being stabilized. The three enzymes showed similar change trend with different effect time. Salinity above 30 might cause great damage to the body with reducing the osmotic pressure regulation and immune defense function. This study provides useful data for future culture of S. constricta.
Key words:  Sinonovacula constricta juvenile  High salt stress  LC50  Enzyme activities
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