| 摘要: |
| 为探讨在不同程度的盐度变化下对驼背鲈（Cromileptes altivelis Valenciennes ♀）与鞍带石斑鱼（Epinephelus lanceolatus ♂ ）的杂交品种（鼠龙斑幼鱼）摄食、抗氧化和消化生理的影响，本实验设置盐度突变组：5、10、15、20、25、30 ppt（对照组）；盐度渐变组（每天盐度降低5 ppt）。在第0、3、7d时分别取样，测定血清和肝脏中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）活力；胃组织中的胃蛋白酶、肠道中的脂肪酶、淀粉酶活性。结果表明：盐度突变组中，盐度降低幅度越大对酶活性的影响越大，尤其5 ppt -15 ppt，三种抗氧化性酶活力在肝脏中的变化趋势相似，均呈显著升高再降低的趋势，血清中的SOD、CAT在第3、7d活力相似，均显著高于其他同时期各组，MDA在第7d显著升高；三种消化酶活力均显著下降，盐度越低，下降幅度越显著；其他各组的抗氧化性酶和消化酶与对照组相比无明显差异。盐度突变组各组的摄食量均呈下降趋势，尤其5 ppt组最低。盐度渐变组SOD、CAT活力呈持续上升趋势，MDA先上升再下降，胃蛋白酶与脂肪酶均是呈先上升再下降的趋势，淀粉酶持续下降；其摄食量下降后又恢复至胁迫前。综上，鼠龙斑对盐度的适应范围较广，当盐度突变为较低的水平（＜15 ppt）时，对鼠龙斑的抗氧化性及消化生理的影响较大，随着胁迫时间延长可能对鱼体肝脏抗氧化系统具有损害作用，从而影响其生长，因此在实际过程中可通过逐渐降低盐度的方式对鼠龙斑进行驯养，以达到降低应激伤害的作用。 |
| 关键词: 驼背鲈×鞍带石斑鱼杂交子代 盐度胁迫 抗氧化性 消化酶 |
| DOI： |
| 分类号:S965.3 |
| 基金项目:冷温性石斑鱼规模化苗种繁育关键技术引进（The introduction of key techniques for seedling-rearing cool-temperate-water groupers),项目编号：2012DFA30360；中国-东盟海上合作基金（China ASEAN Maritime Cooperation Fund Project） |
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| Effects of Short-term Salinity Stress on Antioxidant and Digestive Physiology of Hybrid Progeny（Cromileptes altivelis Valenciennes ♀× Epinephelus lanceolatus ♂） |
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liuling, chenchao
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(1.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture , Yellow Sea fisheries Research Institute, Chinese Academy of fishery Sciences, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology
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| Abstract: |
| The effects of different salinity on the feeding, antioxidant and digestive physiology of the hybrid cultivars Cromileptes altivelis Valenciennes♀ and Epinephelus lanceolatus♂ were studied. In this experiment, five salinity mutation five groups: 5, 10, 15, 20, 25, 30 ppt; salinity gradient group (salinity decreased by 5 ppt per day) were set up,30 ppt used as control group. The activity of superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) in serum and liver, lipase and amylase in intestinal canal and the pepsin in stomach tissue were measured at 0, 3 and 7 d; respectively. The results showed that the effects of salinity on enzyme activities increased with the decreasing salinity in the salinity mutation groups, especially 5-15 ppt group. The changes of the three antioxidant enzymes in liver were similarly, which was increased at first and then decreased. The activity of SOD and CAT in serum was significantly higher than that in other groups at the 3rd and 7th day, and the activity of MDA was significantly increased at the 7th day. The activities of the three digestive enzymes were decreased significantly, and the lower salinity decreased the more significant change. There was no significant change in antioxidant and digestive enzymes between other groups and control group. There was a decreasing trend of food intake in salinity mutation groups, with the lowest value of food intake in 5 ppt group. The activities of SOD and CAT increased gradually and the activity of MDA increased at first and then decreased in the salinity gradient group. The activities of pepsin and lipase increased first, but the activity of amylase decreased continuously. The food intake decreased at first and then returned to the level of control group. In summary, juvenile mussaurus spot can adapt to a wide range of salinity. But when salinity was low (<15ppt), the antioxidant and digestive physiology of juvenile mussaurus spot were significantly influenced. And there may be a damaging effect on the antioxidant system of the liver，even the growth of fish. So salinity gradual decreasing can be used to achieve the required conditions, which can reduce the adverse stress response during salinity acclimation. |
| Key words: Hybrid progeny(Cromileptes altivelis Valenciennes ♀× Epinephelus lanceolatus ♂) salinity stress Antioxidation Digestive physiology |