| 摘要: |
| 为探究黄河口潮间带沉积物夏秋季微生物群落结构的组成及影响因素，采集 8个站位的96个表层沉积物样品，采用高通量测序技术，分析夏秋季沉积物微生物群落结构的组成，并探讨不同环境因子对微生物群落结构的影响。潮间带沉积物中最优势菌门为变形菌门（Proteobacteria），较优势菌门为拟杆菌门（Bacteroidetes）和脱硫菌门（Desulfobacterota）. 秋季和夏季的α多样性存在显著差异，且秋季物种丰富度和均匀度均高于夏季。采用主成分分析（PCA）结合多元方差置换分析（PERMANOVA）研究β多样性，发现夏秋季样本的微生物群落结构存在显著分离现象。通过LEfSe分析发现，夏秋两季存在显著性的属水平类群共计37个，其中夏季9个，秋季28个。夏季和秋季的微生物丰度均与环境因子存在较强的相关性，且不同季节的微生物受不同的环境因子影响，表现出不同的丰度变化。经过FAPROTAX 功能注释，夏秋季丰度均较高的是和氮、硫代谢相关的功能。通过代谢差异进行分析发现，秋季样本中显著上调的主要为自养型代谢功能，而夏季样本中显著上调的则为与分解代谢相关的功能。 |
| 关键词: 黄河口，潮间带沉积物，微生物群落结构 |
| DOI： |
| 分类号: |
| 基金项目:黄河口海域海洋生物多样性调查评价项目(SDGP370000000202302001687) |
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| Microbial community structure of intertidal sediments of the Yellow River estuary and its response to environment from summer to autumn |
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Jiang Shan, Zhao Zhangqi, Jiang Zhichen, Li Xuegang, Zhang Junlong, Zhang Dechao, Sha Zhongli
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Institute of Oceanology, Chinese Academy of Sciences
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| Abstract: |
| To investigate the composition of microbial communities in the intertidal sediments of the Yellow River estuary during the summer and autumn, 96 sediment samples were collected from 8 sampling sites. High-throughput sequencing technology was used to analyze the composition of microbial communities from summer to autumn, and to explore the influence of various environmental factors on the microbial community structure. The most dominant phylum of sediment bacteria was Proteobacteria, and the other dominant phyla were Bacteroidetes and Desulfobacterota. There were significant differences in α diversity between autumn and summer, with both species richness and evenness being higher in autumn than in summer. Principal component analysis (PCA) combined with permutational multivariate analysis of variance (PERMANOVA) was used to study β diversity, and it was found that there was a significant separation in the microbial community structures of samples in summer and autumn. Using LEfSe analysis, a total of 37 significant bacterial taxa at the genus level were identified between the summer and autumn groups, with 9 taxa in summer and 28 in autumn. The microbial abundances in both summer and autumn had strong correlations with environmental factors. Moreover, the microorganisms in different seasons were affected by different environmental factors, showing varying changes in abundance. Through FAPROTAX functional annotation, the functional genes related to nitrogen and sulfur metabolism were found to be abundant in both summer and autumn. In addition, through the analysis of metabolic differences, it was found that the functions that were significantly up-regulated in the autumn samples were mainly autotrophic metabolic functions, while those significantly up-regulated in the summer samples were functions related to catabolism. |
| Key words: the Yellow River estuary, intertidal sediments, microbial community |