| 摘要: |
| 藻类色素通过吸收和传递光能参与光合作用,在不同藻类中色素组成和含量状况也存在明显差异,因此,色素分析是进行藻类光合生理研究和藻种辅助鉴定的重要手段,在浮游植物类群组成和生物量评估等生态学研究中也有广泛应用。针对藻类色素分析,目前已经建立了多种色素提取和分析方法。本研究对比分析了五种细胞破碎色素提取方式(超声处理、手动研磨、冷冻研磨、手动研磨+超声处理、冷冻研磨+超声处理)对微藻和大型藻中色素提取的效果,结果表明：(1)超声处理对具有坚硬硅质细胞壁的硅藻色素提取效果更好,而研磨或超声处理对不具细胞壁的甲藻色素提取效果相似。(2)五种提取方式对两种大型藻中色素的提取效果存在较大差异,在铜藻(Sargassum horneri)色素的提取中尤为明显。对浒苔(Ulva prolifera)中色素的提取效果手动研磨优于冷冻研磨,而冷冻研磨对铜藻色素的提取效果更好,若辅以超声处理可以更好地促进铜藻色素提取。(3) 综合来看,冷冻研磨+超声处理对于微藻和大型藻样品中色素提取的效果最好。该提取方式操作简便、高效省时,在今后藻类色素分析工作中应进一步推广应用。 |
| 关键词: 色素 提取方式 微藻 大型藻 冷冻研磨 |
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| 基金项目:国家重点研发项目（2023YFE0106200）；自然科学基金广西联合基金重点项目（U20A20104） |
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| A Comparative Analysis of Extraction Methods for Algal Pigments |
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SONG Yijia1,2, KONG Fanzhou1, WANG Jinxiu1, YU Rencheng1,2
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1.Institute of Oceanology,Chinese Academy of Sciences;2.University of Chinese Academy of Sciences
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| Abstract: |
| Algal pigments participate in photosynthesis by absorbing and transferring light energy. There are also obvious differences in the composition and content of pigments among different algae. Therefore, pigment analysis is an important approach for photosynthetic physiology study and algal taxa identification, and has been widely applied in ecological studies on phytoplankton community composition and biomass assessment. Many methods have been developed for the extraction and analysis of algal pigments. In this study, five cell disruption and pigment extraction methods—sonication, manual grinding, freeze grinding, manual grinding combined with sonication, and freeze grinding combined with sonication, were compared in extraction and analysis of pigments from both micro- and macro-algae. The major findings are as follows: (1) Sonication was particularly effective in extracting pigments from diatoms with rigid silica cell walls, while both grinding and sonication treatments were effective in extracting pigments from dinoflgaellates without cell wall. (2) The five methods had notable differences in pigment extraction from two species of macroalgae, especially brown alga Sargassum horneri. The manual grinding was more effective than freeze-grinding in extracting pigments from green alga Ulva prolifera, while freeze-grinding was more effective for S. horneri. Sonication treatment could further enhance pigment extraction from S. horneri. (3) Among the five methods, freeze grinding combined with sonication was proved to be the most effective method for pigment extraction from both micro- and macro-algal samples. Such a protocol is easy to operate, efficient, and time-saving, which can be widely applied in future studies. |
| Key words: pigment Extraction method Microalgae Macroalgae Freeze-grinding |