| 摘要: |
| 八放珊瑚作为深海海山生态系统中的关键类群，对维持深海生物多样性具有重要意义。然而，由于传统深海调查技术的限制，目前对深海海山八放珊瑚多样性的研究仍不充分。本研究利用环境DNA宏条形码技术结合影像技术对卡罗琳海山的八放珊瑚多样性进行综合分析，旨在分析环境DNA技术在海山调查中的可行性。通过环境DNA技术，共检测到65个分子种，涵盖2目、11科、19属；而影像技术则识别出29个形态种，涉及2目、6科、9属。两种方法均成功检测到热带西太平洋深海常见的八放珊瑚类群，如金柳珊瑚科、角柳珊瑚科、丑柳珊瑚科和棘柳珊瑚科，但在群落结构和物种组成上存在差异。环境DNA技术在检测隐匿物种和稀有类群方面表现出更高的灵敏度，而影像技术则在物种定量和形态特征分析上更具优势。本研究表明，环境DNA与影像技术的协同应用能够显著提升深海八放珊瑚多样性评估的全面性和可靠性，为深海生物多样性研究提供了新的技术途径。 |
| 关键词: 环境DNA，八放珊瑚，卡罗琳海山，宏条形码技术，影像技术 |
| DOI： |
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| 基金项目:国家自然科学基金(42176128、41930533和42306102)；中国科学院战略性先导科技专项(XDB42000000) |
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| Using environmental DNA and imaging techniques to study coral diversity on the Caroline Seamounts |
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Zheng xiao yu1, Xu yu1, Xu kui dong1, Lei yanli2, Zhan zi feng1
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1.Laboratory of Marine Organism Taxonomy and Phylogeny, Chinese Academy of Sciences;2.Laboratory of Marine Organism Taxonomy and Phylogeny, Qingdao Key Laboratory of Marine Biodiversity and Conservation, Institute of Oceanology, Chinese Academy of Sciences
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| Abstract: |
| Octocorals are a key group in deep-sea seamount ecosystems and play a significant role in maintaining deep-sea biodiversity. However, due to limitations in traditional deep-sea survey technologies, studies on the diversity of octocorals in deep-sea seamounts remain insufficient. This study utilized environmental DNA (eDNA) metabarcoding combined with imaging techniques to conduct a comprehensive analysis of octocoral diversity on the Caroline Seamount, aiming to evaluate the feasibility of eDNA technology in seamount surveys. Through eDNA analysis, we detected 65 molecular species, covering 19 genera in 11 families and 2 orders; in contrast, imaging techniques identified 29 morphospecies, covering 9 genera in 6 families and 2 orders. Both methods successfully detected common deep-sea octocoral groups in the tropical Western Pacific, such as Chrysogorgiidae, Keratoisididae, Primnoidae, and Acanthogorgiidae, but revealed differences in community structure and species composition. eDNA technology demonstrated higher sensitivity in detecting cryptic and rare species, while imaging techniques showed greater advantages in quantitative analysis and morphological characterization. This study demonstrates that the integration of eDNA and imaging techniques can significantly enhance the comprehensiveness and reliability of deep-sea octocoral diversity assessments, providing a new technical pathway for deep-sea biodiversity research. |
| Key words: Environmental DNA, Octocorallia, Caroline Seamount, metabarconding, imaging technique |