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| 亚汕酸修饰羧甲基壳聚糖纳米粒固定化菠萝蛋白酶的研究 |
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谭玉龙, 刘晨光, 刘成圣, 于乐军
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中国海洋大学海洋生命学院
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| 基金项目:山东省优秀中青年科学家科研奖励基金资助 |
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| STUDY ON LINOLEIC ACID MODIFIED CARBOXYMEHYL CHITOSAN NANOPARTICLES FIXATION BROMELAIN |
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Tan Yulong, Liu Chenguang, Liu Chenshen, Yu Lejun
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College of Marine Life Sciences,Ocean University of China
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| Hydrogel nanoparticles can be prepared by using linoleic acid (LA) modified carboxymethyl chitosan (CMCS) after sonication. Bromelain can be loaded onto nanoparticles of LA-CMCS. Factors affecting the activity of the immobilized enzyme, including temperature, storage etc., were investigated in this study. The results showed that the stability of bromelain for heat and storage was improved after immobilization on nanoparticles. The Michaelis constant (Km) of the immobilized enzyme, was smaller than that of free enzyme which indicated that the immobilization could promote the stability of the enzyme and strengthen the affinity of the enzyme for the substrate. |
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