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| Cloning and Analysis of Core Sequence of Tegillarca Granosa Mn-Superoxide Dismutase Gene |
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Du Lili, Li Taiwu, Su Xiurong, Li Dengfeng, Pan Safang
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Faculty of Life Science and Biotechnology,Ningbo Uciversity
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| Special primers were designed based on the sequence information of superoxide dismutase genes reported in the Iiterature and were used for RT-PCR and rapid amplification of cDNA ends(RACE) to clone Tegillarca granosaMn-superoxide dismutase gene successfully.The length cDNA of Tegillarca granosaMn-superoxide dismutase gene was of 635bp by including a 3'untranslated region(UTR)of 230bp and an open reading frame(ORF) of 4.5bp encoding a polypeptide of 135 amino acids. TheTegillarca granosaMn-superoxide dismutase gene shares 66%~73% homology with Mizuhopecteb yessoensis's,Danio rerio's,Xenopus Laevis's,Human's,Callinectes sapidus'sSOD CDS, while the corresponding amino acids shares 68%-78% homology and 80%~89% comparability. These indicate the SOD gene is high evolutional conservation in animals. |
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