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盐度调控对花鲈生长相关基因表达的影响
张沛,迟美丽,温海深,钱焜,倪蒙,张亚晨,黄政举,宋志飞,柴森浩
作者单位E-mail
张沛 中国海洋大学水产学院 pei325@126.com 
迟美丽 中国海洋大学水产学院  
温海深 中国海洋大学水产学院 13853270722@163.com 
钱焜 中国海洋大学水产学院  
倪蒙 中国海洋大学水产学院  
张亚晨 中国海洋大学水产学院  
黄政举 中国海洋大学水产学院  
宋志飞 中国海洋大学水产学院  
柴森浩 中国海洋大学水产学院  
摘要:
生长激素受体(GHR)作为GH/IGF轴的中心环节,在内分泌调控中发挥重要作用。本实验采用cDNA末端快速扩增法(RACE)技术克隆出花鲈GHR1和GHR2的cDNA全长序列。急性低盐度调控实验设为海水组、半海水组和淡水组。测定了急性低盐度调控24h、48h、96h、144h、192h后,花鲈肝中GHRs、IGF-1及垂体GH表达情况。结果表明:GHR1 cDNA全长序列2436bp,编码637个氨基酸;GHR2 cDNA全长序列2940bp,编码582个氨基酸。GHR1 与GHR2 由信号肽、胞外区、跨膜区、胞内区组成,且结构存在差异。脑、肾、鳃中GHR1表达明显高于GHR2;而在肌肉、垂体、肝脏、盲肠、胸腺、心脏中,GHR2表达明显高于GHR1。24h时,各组GHR1表达不变,GHR2、GH、IGF-1显著下降。之后,相对于海水组,淡水组和半海水组GHRs和IGF-1表达升高,而GH下降,GH与GHR负相关。据结果推测,花鲈GHR2可能为SL受体,GH/IGF轴参与低渗调控可能是通过增加GHRs,进而激活下游IGF-1表达而实现。
关键词:  花鲈  盐度  生长激素受体  生长激素  胰岛素样生长因子1
DOI:10.11693/hyhz20140400102
分类号:
基金项目:国家科技攻关计划
EFFECTS OF SALINITY ON THE EXPRESSION OF GROWTH RELATED GENES IN JAPANESE SEABASS(LATEOLABRAX JAPONICUS)
ZHANG Pei,CHI Meili,WEN Haishen,QIAN Kun,NI Meng,ZHANG Yachen,HUANG Zhengju,SONG Zhifei,CHAI Senhao
Abstract:
Growth hormone receptors(GHRs), as the center of GH/IGF axis, play key roles in individualendocrine. cDNA sequence of Japanese seabass(Lateolabrax japonicus)two GHRs was cloned by rapid amplification of cDNA cends (RACE). Acute salinity regulation experiment contained three group: seawater group,semi-seawater group and fresh water group. Hepatic GHRs, Insulin-like growth factor(IGF-1) and hypophyseal growth hormone(GH) were determined after 24h, 48h, 96h, 144h, 192h by qPCR.GHR1and GHR2 were2436bp and 2940bpin length, which encoded 637 and 582 amino acidrespectively. GHR1 and GHR2 contained different putative signal peptide, extracellular domain, transmembrane domain and intracellular domain. Expression level of GHR1 were higher than GHR2’s in brain, kindey and gill, while it is contrary in muscle, pituitary, liver, caecum, thymus and heart.GHR1mRNA was Constant, while the levels of GHR2, GH and IGF-1 decreased at 24h. Compared with seawater group,there were higher expression of GHRs, IGF-1 and lower expression of GH in semi-seawater group and fresh water group with following salinity regulation. These results suggested GHR1 ofJapanese seabasswas somatolactin receptorprobably. GH/IGF axis participates in osmoregulation, probably in the way of improving the levels of GHRs, furthermore activated the expression of IGF-1.
Key words:  Lateolabrax japonicas  Salinity  growth hormone receptor  growth hormone  insulin-like growth factor 1
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