| 摘要: |
| 于1992年4月—1992年6月以扁藻为材料进行半抑制浓度三苯基氯化锡处理72h实验，用透射电镜观察中毒状态扁藻细胞超微结构的变化。结果表明，线粒体发生水肿，内脊局部瓦解，基质内出现由双层膜包围的同心质膜轮；叶绿体内光合片层的网状结构被破坏，光合片层上下重叠成厚块状；蛋白核中央髓部肿胀，淀粉鞘破裂成多角形淀粉板，并分散在髓部周围；叶绿体基质中出现大量淀粉粒，眼点因受淀粉植挤压而由藻体中下部移至藻体底部。比较各细胞器对TPTC的反应，线粒体和叶绿体最为敏感，细胞核则表现得相当稳定。 |
| 关键词: 三苯基氯化锡 半抑制浓度 扁藻 超微结构 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助，48970274号 |
附件 |
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| EFFECTS OF TRIPHENYLTIN CHLORIED (TPTC) ON ULTRASTRUCTURES OF CELL OF PLATYMONAS sp. |
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Chen Guowei1, Li Yun1, Jiang Ming2, Liu Xiaoyun2
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1.Department of Biology, Ocean University of Qingdao, Qingdao 266003;2.Centre of Analysis, Ocean University of Qingdao, Qingdao 266003
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| Abstract: |
| Electron microscope examination of treated cells of Platymonas sp. cultured in medium contamination by TPTC at concentration of 6.7×10-9 mol/L (EC50) revealed fine structure changes in that cells. The results were as follows: The mitochondria swelled up and its cristae were disintegrated in parts, the concentric membranous worls appeared in the matrix and it was bounded by double membranes; in the chloroplast the reticulate structure of photosynthetic lamellae were damaged and instead of lumping structure due to the lamellae were piled up; the pith of the pyrenoid distended and the starch shell splited up in the several polygonal starch plates around the pith; a large number of starch grains appeared in strome of chloroplast; the stigma moved from the position in the sub-middle of the cell to the bottom of it by the press of starch grains. The nucleus was not morphologically affected.
The mitochondria and chloroplast were the most sensitive in their cytological response to TPTC in the cell; in most cases, the nucleus did not change at all. |
| Key words: TPTC, EC50, Platymonas sp., Ultrastructure |
