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引用本文:朱文杰,汪杰,陈晓耘,扎西次仁,杨韵,宋瑛.发光细菌一新种──青海弧菌.海洋与湖沼,1994,25(3):273-279.
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发光细菌一新种──青海弧菌
朱文杰, 汪杰, 陈晓耘, 扎西次仁, 杨韵, 宋瑛
华东师范大学生物系 上海 200062
摘要:
于1985年8月从青海湖产的青海裸鲤体表分离到70株发光细菌,通过表型特性分析、超氧化物岐化酶的凝胶双向扩散试验,以及G+C摩尔百分比测定进行鉴定分类。结果表明,70株发光细菌在表型特性、免疫学特性等方面彼此高度相似,但与已知的各种发光细菌有显著差异。鉴定为弧菌目的一个新种,定名为青海弧菌Vibrio qinghaiensis sp.nov。典型菌株保存于华东师范大学生物系。
关键词:  发光细菌  弧菌属  青海弧菌  新种  青海湖
DOI:
分类号:
基金项目:国家教育委员会资助,840729/060115号
附件
A NEW SPECIES OF LUMINOUS BACTERIA VIBRIO QINGHAIENSIS SP. NOV.
Zhu Wenjie, Wang Jie, Chen Xiaoyun, Zhaxi Ciren, Yang Yun, Song Ying
Department of Biology, East China Normal University, Shanghai 200062
Abstract:
Seventy isolates of luminous bacteria were isolated in August, 1985 from body surfaces of the fish, Gymnocypris przewalskii, living in Qinghai Lake were subjected to extensive tests for their characterization. Antisera were applied to their superoxide dismutase and ouchterlony double diffusion experiment was carried out with some of the isolates. The bioluminescent spectra of these isolates in vivo were also researched.

All of the isolates were very similar in their phenotype, and can ferment D-glucose. Their growth was inhibited by 0/129. They had a polar flagellum, and were facultative anaerobic and Grame-negative. The results indicated that they were different from previously identified luminous bacteria species in some properties. They can grow well and emit light in media without sodium ions or any organic growth factors, can grow in media with 1% - 8% of salt concentration and pH of 6.5 - 11. Their optimal growth salt concentration was 1%, and optimal growth Ph was 9. They can accumulate PHB in their cells as an intracellular reserve product, yield yellow pigment in solid media at later stage of growth, and utilize many kinds of organic compounds as sole or principal sources of carbon and energy. They had a G + C content in their DNA of 47.1 mol%. The seventy isolates were assigned to the genus Vibrio and named Vibrio qinghaiensis sp. nov.. Strain Q-67 was designated as the type strain of this new species differing from the most related species V. harveyi as follows: growth at 4°C, growth in media without sodium ions, optimal growth salt concentration being 1%, ability to produce yellow pigment, to accumulate PHB in their cells, and to utilize (β-hydroxybutyrate, L-leucine, L-tyrosine, sorbitol, but not propionate.

The type strain Q-67 is preserved in the Department of Biology, East China Normal University.

Key words:  Luminous bacteria, Vibrio, Vibrio qinghaiensis sp. nov., Qinghai Lake
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