引用本文:	马红,高振泮,翟小京,娄清香,马桂芝,邓文涛,毛大璋,李良壁.不同植物光合膜中镁离子诱导荧光同膜表面电荷变化相关性的比较研究.海洋与湖沼,1994,25(5):515-520.

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不同植物光合膜中镁离子诱导荧光同膜表面电荷变化相关性的比较研究
马红¹, 高振泮¹, 翟小京¹, 娄清香², 马桂芝¹, 邓文涛¹, 毛大璋¹, 李良壁¹
1.中国科学院海洋研究所青岛 266071;2.中国科学院植物研究所北京100044

摘要:

用水培的大麦苗及1992年夏、秋在青岛汇泉湾采集的大叶藻和利松藻制备叶绿体膜，观测了Mg²⁺诱导它们的PS－II Chl a荧光和膜表面电荷变化之间的相互关系。发现在大麦和大叶藻中，Mg²⁺诱导荧光的增高与其诱导膜表面电荷密度的降低平行相关；而在刺松藻中，则无类似的相关性变化。这不仅证明在大麦和大叶藻的叶绿体膜中阳离子对激发能分配的调节作用受膜表面静电性质的控制，而在刺松藻的叶绿体膜中则与膜表面静电性质的变化无关；揭示这两种不同的调节机理与植物的生长环境无关，而可能取决于植物的进化程度。

关键词: 叶绿体膜光系统捕光叶绿素a／b-蛋白复合体

DOI：

分类号:

基金项目:中国科学院海洋研究所调查研究报告第2340号。中国科学院海洋研究所实验海洋生物学开放研究实验室资助，923011号

附件

COMPARATIVE STUDY ON THE RELATIONSHIP BETWEEN Mg²⁺-INDUCED FLUORESCENCE CHANGE AND Mg²⁺-INDUCED THYLAKOID SURFACE CHARGE ALTERATION IN THE CHLOROPLASTS ISOLATED FROM DIFFERENT PLANTS

Ma Hong¹, Gao Zhenpan¹, Zhai Xiaojing¹, Lou Qingxiang², Ma Guizhi¹, Deng Wentao¹, Mao Dazhang¹, Li Liangbi¹

1.Institute of Oceanology Academia Sinica, Qingdao 266071;2.Institute of Botany, Academia Sinica, Beijing 100044

Abstract:

Chloroplasts isolated from barley (Hordeum valgare L.) seedling grown in liquid solution and from Zostera marina and Codium fragile collected in summer and autumn of 1992 at Huiquan Bight of Qingdao Were used in this study on the relationship between Mg²⁺-induced Chl a fluorescence change and Mg²⁺-induced thylakoid surface charge alteration in chloroplasts. Result shows that Mg²⁺-induced Chl a fluorescence intensity increase from PS-II closely correlated with Mg²⁺-induced electric charge density decrease on the outer surface of the thylakoid in the chloroplasts from barley and Zostera marina. However, under the same conditions the correlation was not noted in the chloroplast from Codium fragile. These experimental results not only demonstrated that the regulating effect of the cation on excitation energy distribution between two photosystems was controlled by the electrostatic property of the thylakoid surface in the chloroplasts of barley and Zostera marina and not controlled by the electrostatic property of the thylakoid surface in the chloroplast of Codium fragile, but also illustrated that these two kinds of regulating mechanisms did not depend on the growing environment of plants while they might be associated with the evolutional level of plants.

Key words: Chloroplast Photosystem, Light-harvesting chlorophyll a/b-protein complex