| 摘要: |
| 于1986－1990年，在大连凌水养殖四场采集海带，对磷酸烯醇式丙酮酸羧激酶在海带不同部位、不同发育时期及不同外界条件下活性的变化予以研究。结果表明，该酶是海带体内唯一重要的催化CO2暗固定的酶；此酶活性由海带基部、中部至顶端逐渐减弱；酶活性在用正常水温预处理藻体时最高；正常盐度时酶的活性高于非正常盐度的酶活性；增加NH4+浓度可提高酶的活性。 |
| 关键词: 海带 磷酸烯醇式丙酮酸-羧激酶 CO2暗固定 活性 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助，386072号 |
附件 |
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| STUDIES ON THE PHOSPHOENOLPYRURATE CARBOXYKINASE OF LAMINARIA JAPONICA |
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Chen Minzi, Hou Hesheng, Yao Nanyu, Xu Zhiming, Li Jianzhi
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Department of Biology, Liaoning Normal University, Dalian 116022
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| Abstract: |
| Phosphoenolpyrurate Carboxykinase (PEP-CK) activities of different parts, developmental periods, and conditions of marine brown algae Laminaria japonica collected from the Ling Shui breeding station were studied from 1986 to 1990. The results showed that PEP-CK is the sole important enzyme which catalyzed light independent fixation of carbon dixoide in L. japonica. PEP-CK activity in basal part of the thallus was high, and decreased gradually toward the apical part. The enzymic activity in the young developmental period was higher than that in the mature period.
There were some tendencies that some environmental factors might affect the PEP-CK activity, that is, the activities at/in normal seawater temperature and salinity were higher than that at/in abnormal ones. Increasing the content of NH4+ in madium could also promote PEP-CK activity. |
| Key words: Laminaria japonica, Phosphoenolpyrurate carboxykinase, Light independent fixation of CO2, Activity |
