| 摘要: |
| 于1989年9－11月分别在中国科学院水生生物研究所和天津轻工部制盐研究所取得盐藻Dunaliella sp. HB558和Dunaliella salina 1009。这两个藻种的光合放氧-光强曲线，光系统Ⅰ、光系统Ⅱ活性-温度曲线及甘油产量的结果表明，其光合作用的光饱和点皆为220 μmol/(m2?s)，并可忍受40°C的温度；当温度为45°C时，D．sp．HB558的光合放氧明显受到抑制，光系统II活性下降，但对从D. salina 1009则无影响; D. sp. HB558在10 - 40℃下的光合放氧速率及甘油产量都高于D. salina 1009。培养这两种藻的最适温度为30 - 35°C。 |
| 关键词: 盐藻 光饱和点 光系统Ⅰ 光系统Ⅱ 光合放氧 甘油 |
| DOI： |
| 分类号: |
| 基金项目:中国科学院华南植物研究所所长基金资助，920350号 |
附件 |
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| COMPARATIVE STUDY ON PHOTOSYNTHETIC PROPERTY OF TWO SPECIES OF DUNALIELLA |
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Peng Changlian1, Lin Zhifang1, Sun Guchou1, Shi Dingji2
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1.South China Institute oI Botany, Academia Sinica, Cuangzhou 510650;2.Institute of Botany, Academia Sinica Beiiing 100044
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| Abstract: |
| Dunaliella sp. HB558 and Dunaliella salina 1009 were supplied by the Institute of Hydrobiology, Academia Sinica, Wuhan and Salt Research Institute, Tanggu, Tianjin, respectively. The different temperature photosynthesis light-response curves of Dunaliella were obtained by measurement of O2 evolution using a Clarktype oxygen electrode. The effect of temperature on activities of photosystem I and photosystem II as well as the respiratory rate were also detected by the same technique.
The light saturation point of photosynthesis in these two species of Dunaliella, was 220 μmol/(m2?s). In both species, the photosynthetic rate increased with temperature up to 40°C. The observed inhibition of photosynthetic oxygen evolution of D. sp. HB 558 (but not of D. salina 1009) by high temperature (45°C) was due to the damage of PS II. The photosynthetic oxygen evolution of D. sp. HB 558 was much higher than that of D. salina 1009 at all experimental temperatures (except 45°C).
The glycerol accumulated in cells of D. sp. HB 558 was 172.77 mg/(mg?Ch1.) and of D. salina 1009 was only 70.65 mg/(mg?Chl.). The higher glycerol content of D. sp. HB 558 was due to its high photosynthetic capacity. The maximum photosynthesis to respiration rations of D. sp. HB 558 and D. salina 1009 were at 35°C (6.76) and 30°C (7.72), respectively. The photosynthetic oxygen evolution at 30°C was 73% (D. sp. HB 558) and 71% (D. salina 1009) of that at 35°C. It is suggested that the optimal temperature for cultivating D. sp. HB 558 and D. salina 1009 is 35°C rather than 30°C.
In comparison with D. salina 1009, D. sp. HB 558 showed higher photosynthetic capacity and glycerol content but lower tolerance to high temperature. |
| Key words: Dunaliella, Light saturation point, PS I, PS II, Photosynthetic oxygen, Glycerol |
