| 摘要: |
| 于1985－1987年期间用冷水和热水对采集于青岛的龙须菜（1986年7月）和扁江篱（1985年12月）的琼胶型多糖进行提取。两海藻的热水提取多糖都通过DEAE－Sephadex A50色谱柱用热水和不同浓度Nacl溶液前后进行洗脱分级。龙须菜和扁江蓠多糖的主要级分分别为0.5mol/L和1.0mol/L Nacl洗脱级分。对各级分做化学分析及IR和13C-NMR光谱分析。结果表明，龙须菜多糖由琼二糖、琼胶糖前体和6－OCH3-琼二糖组成；扁江蓠多糖则由琼二糖、6－OSO3--琼二糖和6－OCH3-琼二糖组成。对两海藻的提取多糖及个别级分的抗肿瘤（S180）效果试验结果表明，龙须菜的冷水提取多糖和扁江蓠的热水提取多糖的初筛抑瘤率分别为45％和60%。 |
| 关键词: 龙须菜 扁江蓠 13C-NMR光谱分析 分级 抗肿瘤效果 |
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| COMPOSITION OF POLYSACCHARIDES OF GRACILARIA SJEOSTEDTII KYLIN AND G. TEXTORII (SUR.) De-Toni (RHODOPHYTA) AND THEIR i ANTITUMOR EFFECT |
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Deng Zhifeng,Ji Minghou
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Institute of Oceanology, Academia Sinica, Qingdao 266071
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| Abstract: |
| Two agar type polysaccharides were extracted with hot water and cold water from Gracilaria sieostedtii Kylin and G. textorii (Sun) De-Toni (Rhodophyta) collected on the seashore of Qingdao from 1985 to 1987, and fractionated separately on DEAE-Sephadex A50 chromatographic column by elution with water and various concentration of NaCl solutions. The results showed that the main fraction from G. sieostedtii polysaccharide was 0.5 mol/L NaCl fraction, yielding 41.3% of the total fraction, and that from G. textorii polysaccharide was 1.0 mol/L NaCl fraction, yielding 33.6% of the total fraction. The eluted fractions were analyzed by chemical methods and by IR and 13C-NMR spectroscopic methods. It was clarified that the main component of the 0.5 mol/L NaCl fraction from G. sieostedtii was a mixture of agarose and precursor to agarose, while that of the 1.0 mol/L fraction from G. textorii was a mixture of agarose, 6-OCH3-agarose and 6-OSC3--agarose. The polysaccharides and some fractions from both species were ,tested for their antitumor effect against Sarcoma 180 in rats. The results indicated that the antitumor efficiencies of the polysaccharide extracted by cold water from G. sfeostedtii and of that extracted by hot water from G. textorii were 45% and 60%, respectively. These polysaccharides would be promising' antitumor substances after further study through animal and clinical tests. |
| Key words: Gracilaraia sieoszcdtii, G. textorii, 13C-NMR spectroscopic analysis, Fraction, Antitimor effect |
