| 摘要: |
| 于1986年1月-11月用高效液相色谱法分析胶州湾10个站位表层和底层海水中海解游离氨基酸（DFAA）的组成、含量的分布及其季节变化。结果表明，7个站位的表层海水中月平均总溶解游离氨基酸（TDFA）含量范围为1.24－2.28 μmol/L。多数站位在2月份处于最低值（0.4 μmol/L），11月份达最高值（5.0 μmol/L）。所测氨基酸中，Glu，Gly,Arg，Leu，Orn，Ser等占优势．湾内东部几个站位表层水中DFAA含量明显地高于其他站位。各站位的溶解结合氨基酸（DCAA）含量一般都高于DFAA的含量，而且两者的氨基酸组成也有差异。几个站位的底层水中的TDFAA含量，除个别外，一般都低于表层。室内实验表明，DFAA最易受生物过程的影响。 |
| 关键词: 反相高效液相色谱（HPLC）法 溶解游离氨基酸（DFAA） 溶解结合氮基酸（DCAA） 总溶解游离氨基酸（TDFAA） 胶州湾 |
| DOI： |
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附件 |
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| STUDIES ON DISSOLVED AMINO ACIDS IN SEAWATER OF JIAOZHOU BAY |
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Lu Tiansheng, Ji Minghou
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Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071
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| Abstract: |
| The distribution and seasonal variation of dissolved free amino acids (DFAA) in the surface and bottom seawater of Jiaozhou Bay at ten stations from Jan. to Nov. in 1986 were investigated using reverse-phase HPLC method.
The monthly average contents of total dissolved free amino acids (TDFAA) in surface seawater at seven station from Jan. to Nov. range from 1.24 to 2.28 μmol/L. Most stations exhibit the lowest values (0.4 μmol/L) in Feb., generally lower than 1.0 μmol/L. The highest value (5.0 μmol/L) always appears in Nov.
Among all DFAAs determined, Glu, Gly, Arg, Leu, Orn and Ser are the most dominant amino acids. The average contents of neutral, basic and acidic amino acids to total amino acids in surface seawater at C5 station are 55%, 30% and 15%, respectively. The DFAA contents in eastern stations of Jiaozhou Bay near the coast of the city give much higher values in surface seawater than in other stations.
The contents of dissolved combined amino acids (DCAA) are generally higher than those of TDFAA and both compositions of amino acids are somewhat different. The TDFAA contents in the bottom water are usually lower than those in surface water.
The decomposition experiment of particulate organic matter (POM) shows that in the course of decomposition, the DFAA and DCAA are firstly and dominantly released and easily influenced by biological process. |
| Key words: Reverse-phase HPLC method, Dissolved free amino acid (DFAA), Dissolved combined amino acid (DCAA), Total dissolved free amino acid (TDFAA), Jiaozhou Bay |
