摘要: |
于1992年4月在青岛前海栈桥湾采集野生的裙带菜,并在室内分离和培养雌、雄配子体克隆,利用营养生长的配子体材料研究日光照时数(DH)对配子体发育的影响。结果表明,连续光照可以有效地阻止配子体发育,配子体只进行营养生长(VG),形成卵囊的配子体细胞所占的比例为零,雌配子体细胞呈不规则圆形,多个细胞聚集成簇状,雄配子体则长成丝状体;随着DH的减少,发育的配子体细胞数比例逐渐上升,至DH为12时达最高,为63%,之后随着DH的减少,发育的比例又继续下降;当DH为8,15,22时,配子体细胞发育的比例分别为35%,42%和3.8%;不同的OH除了影响配子体细胞发育比例以外,还对配子体的细胞形态以及幼抱子体的形态也有不同的影响。 |
关键词: 裙带菜 配子体 光周期 生长节奏 |
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INFLUENCE OF DAYLENGTH HOURS ON GAMETOGENESIS OF U. PINNAHFIDA (HAR.) SUR. |
Pang Shaojun
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Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071
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Abstract: |
Wild sporophtes of U. pinnatifida were sampled at Zhanqiao bay, Qingdao in April 1992- Male and female gametophyte clones were isolated and cultured in lab. Vegetative gametophytes were used to study the effects of different daylength hours (DH) on gametogenesis. The results indicated that continuous light can effectively inhibit gametogenesis Gametophytes grew vegetatively. The percentage of ooganium was zero. Optimum DH was 12, when the percentage of ooganium reached highest level - 63%. DH higher or lower than 12 resulted in decreased gametogenesis percentage. Gametogenesis percentages were 35%, 42% and 3.8%, when DH were 8, l5 and 22 respectively. Observation results in sporophyte culturing experiments under different DH showed that DH also influences the structure of sporophyte morphology. Some aspects related to different responses of gametophyte of U. pinnatifida to daylength hours are also discussed in this paper. |
Key words: Undaria pinnatifida, Gametophyte, Photoperiods, Growth rhythms |