| 摘要: |
| 采用1984年在美国加利福尼亚大学研究和培育成功的盐泽螺旋藻变种，经扫描电镜、生物量测定和生化分析，进一步研究其基本特性。结果表明，其藻丝呈紧密的逆时针向等宽螺旋结构、它在36°C的平均生长速率（K）= 0.12,产量可达48.4 g/(m2*d)（干重）；蛋白质含量平均为50.47％；氨基酸17种；铁超氧物歧化酶含量为0.05 mg/g（鲜重）；β-胡萝卜素含量为3.5 mg/g（干重）。以上结果证明，盐泽螺旋藻变种具有优良的性状，可以应用于大规模生产开发。 |
| 关键词: 盐泽螺旋藻变种 生长速率 蛋白质 超氧物歧化酶 β-胡萝卜素 |
| DOI： |
| 分类号: |
| 基金项目:浙江省自然科学基金资助项目，288254号 |
附件 |
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| THE GROWTH AND BIOCHEMICAL CHARACTERISTICS OF SPIRULINA SUBSALSA VAR. |
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Qian Kaikian, Hou Shengying
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Department of Bioscience and Technology, Zhejiang University, Hangzhou 310027
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| Abstract: |
| Some growth and biochemical characteristics of Spirulina subsalsa var. Crassior virieux determined by Qian et al. in their research by scanning electron microscopy, growth rate measurements, and biochemical analyses at the University of California, San Diego in 1984. The procedures for biochemical analysis included determination of the composition and content of amino acids by Backman autoanalyzer, the purification, content, and activity of superoxide dismutase (SOD) by column chromatograpy, Bradford's method (1976), and Beauchamp’s method (1971) respectively, the content of β-carotene by paper chromatograpy. Results showed that the trichome was twined into a tight and equally wide anti-clockwise helix very different in morphology from Spirulina platensis and Spirulina maxima. The biomass of this alga increased about 10 fold (from 0.1 280 g/L to 1.4 360 g/L) in nine days under in temperature of 36°C, that is, the growth rate (K) was 0.12. The yield of this alga at 36°C may reach 48.4 g/(m2*d) (dry weight), while that of Spirulina platensis may be as high as 40 g/(m2*d) under warm conditions. The protein content was 50.47% algal dry weight. Seventeen kinds of amino acids were measured; the content of Fe-SOD was 0.05 g/g (algal fresh weight) and the SOD activity value was 5577.7 U/mg; the content of β-carotene was 3.5 mg/g (algal dry weight) twice that of Spirulina platensis. The study results indicated that Spirulina subsalsa var. is a promising species for mass culture. |
| Key words: Spirulina subsalsa var., Growth rate, Protein, Superoxide dismutase, β-carotene |
