| 摘要: |
| 于1992－1993年5月和10月，在胶州湾捕捞中国对虾亲虾，9月在莱州和胶南采集的人工养殖中国对虾，采用双缩肥法测定其血淋巴中的血清总蛋白含量，用0－TB法测定血清葡萄糖含量。并研究注射灭菌海水、海洋弧菌后，对中国对虾血清总蛋白含量和血清葡萄糖含量的影响规律。结果表明，总蛋白含量秋天正在发育期的亲虾为132 mg/ml，春天已产卵亲虾的为90 mg/ml；向中国对虾腹部肌肉分别注射灭菌海水、弧菌在24, 48h后，从（表面未发现异常变化的）中国对虾围心腔抽取血淋巴，测定其血清蛋白含量和葡萄糖含量，未发现明显差异。但已感染黑斑病的中国对虾，其血清蛋白含量为54 mg/ml，血清葡萄糖含量为641 μg/ml，说明感染黑斑病的虾，血清蛋白含量明显减少，而葡萄糖的含量急剧增大：这可以作为中国对虾感染黑斑病的一种血液化学监测指标。 |
| 关键词: 中国对虾 血淋巴 血清蛋白 血清葡萄糖 黑斑病 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目，3927054号 |
附件 |
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| STUDY ON THE CONTENTS OF SERUM PROTEIN AND GLUCOSE IN THE HAEMOLYMPH OF THE SHRIMP PENAEUS CHINENSIS |
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Luo Rixiang
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Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071
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| Abstract: |
| Wild (captured in May and October) and cultured (collected in September) shrimp Penaeus chinensis were used in this study on the total serum protein and total glucose in their haemolymph. The total serum protein was measured by Biuret reaction, and the total serum glucose, by O-TB. The efects on the contents of the above-two biochemicals caused by injection of sterilized seawater and marine Vibrio sp. (0.05×108 cell/ml) were investigated. The total serum protein was 132 mg/ml in the not-spawning adult shrimp collected in autumn; and 90 mg/ml in the spawning shrimp collected in spring. No variation of the contents of serum protein and glucose in the. haemolymph from the pericardial sinus was observed 24 and 48 hours after intramuscular injection of sterilized seawater and Vibrio in the abdomen. The contents of serum protein and glucose were 54 mg/ml and 64l μg/ml respectively in the shrimps infected by black spot disease. The results showed P. chinensis infection by black spot disease is symptomized by obvious decrease of serum protein content accompanied by increase of serum glucose content. |
| Key words: Penaeus chinensis, Haemolymph, Serum protein, Serum glucose, Black spot disease |
