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引用本文:高洪峰,纪明侯,曹文达,韩丽君.用β-琼胶酶和NMR光谱法研究多管藻多糖的寡糖结构.海洋与湖沼,1996,27(5):505-510.
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用β-琼胶酶和NMR光谱法研究多管藻多糖的寡糖结构
高洪峰, 纪明侯, 曹文达, 韩丽君
中国科学院海洋研究所 青岛 266071
摘要:
于1990年3月用β-琼胶酶对多管藻的冷水提取多糖进行酶解,酶解液通过DEAE-SephadexA25和Bio-Gel P6色谱柱分离,分离出2个带电荷的寡糖A-Ⅰ和A-Ⅱ.经1H-NMR和13C-NMR光谱法分析,确定结构式分别为61,22-二-O-甲基-63-硫酸基-新琼四糖和61,22-二-O-甲基-63,65-二硫酸基-新琼六糖,证明它们构成多管藻多糖分子的单位组分。
关键词:  β-琼胶酶  多管藻  带电荷琼胶寡糖  新琼四糖  新琼六糖
DOI:
分类号:
基金项目:国家自然科学基金资助项目,3870137号
附件
STUDIES ON THE STRUCTURE OF OLIGOMERS FROM THE POLYSACCHARIDE OF POLYSIPHONIA URCEOLATA (RHODOPHYTA) USING β-AGARASE AND NMR SPECTROSCOPY
Gao Hongfeng, Ji Minghou, Cao Wenda, Han Lijun
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071
Abstract:
The polysaccharide extracted by cold water from Polysiphonia urceolata collected on March 1990, in Qingdao, was hydrolyzed using β-agarase from Pseudomonas atlantica. The mixture after hydrolysis was precipitated by adding 95% ethanol solution, then the upper clear solution was evaporated in vacuo. This ethanol concentrate of enzyrnic hydrolyzate was chromatographed on DEAE-Sephadex A25 and then Bio-Gel P6 columns. Two charged agarooligomers A-I and A-II were isolated from the eluents. Their degrees of polymerization were estimated by 1H-NMR spectra to be 2 and 3, respectively. Two oligomers gave clear resonance signals at 3-41 ppm and 3.52 ppm in 1H-NMR, which showed the presence of methyl groups on C2 of L-galactose and C6 of D-galactose, respectively. A strong signal at 4.18 ppm indicated that a sulfate group links with C6 of D-galactose. The structure of the oligomers A-I and A-II was deduced by the assignment of chemical shifts in 1H-and 13C-NMR spectra to be 61, 22-di-nethyl-meoagarotetraose-63-sulfate and 61, 22-di-methyl-neoagarohexaose-61, 65-disulfate, respectively, which constitute the unit component of the polysaccharide of Polysiphonia urceolata.
Key words:  β-agarase, Polysiphonia urceolala, Charged agarooligomer, Neoagarotetraose, Neoagarohexaose
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