Shrimps(Penaeus chinensis) averaging 9.02cm in body length as the experimental material were cultured in our laboratory from June to September, 1996. The protein content of hepatopancreas, stomach and midgut were measured by Biuret reaction. The protease activity was measured by Folin-Phen reaction; the amylase and cellulose activity by the Somogyi method The shrimps were fed with dry supplemented added with 10% beet dregs or 10% various mould fermented beet dregs; and the effects of these two kinds of feed activities were studied. The results were as follows:
1. The protein content in hepatopancreas, stomach and midgut was 7.95 - 9.30 μg, and was not affected by the diet supplemented with 10% beet dregs or 10% beet dregs fermented by Trichoderma koningii and Aspergillus niger.
2. The protease activity in hepatopancreas, stomach and midgut was 0.892 μg/min in the shrimp fed with the diet supplemented with 10% beet dregs; and 0.222 μg/min for the shrimps fed with 10% beet dregs fermented by T. koningii and A. niger.
3. The amylase activities were increased in the shrimps fed the diet supplemented with 10% beet dregs or 10% beet dregs fermented by T. koningii and A. niger and 3.349 μg/min, 3.711 μg/min respectively, and the control was 2.66 μg/min respectively.
4. The cellulase activity of the shrimps was increased only by the diet supplemented with 10% beet dregs fermented by T. koningii and A. niger, and was 0.451 μg/min. The cellulase activity of the shrimps fed with diet supplemented with 10% beet dregs was 0.1106 μg/min, and was 0.2043 μg/min in the control. |