| 摘要: |
| 于1996年10月在中国科学院海洋研究所选取入工养殖中国对虾（平均体长9.02cm）。以双缩脲法测定消化器官的蛋白含量；福林-酚试剂法测定蛋白酶活力；Somogyi比色定糖法测定淀粉酶和纤维素酶活力，同时研究中国对虾配合饵料中添加10％的甜菜渣或10％经不同方法处理的甜菜渣时，对消化器官蛋白酶、淀粉酶、纤维素酶活力变化的影响。结果表明，在饵料中添加10％甜菜渣或经不同方法处理的甜菜渣饲喂中国对虾，没有影响消化器官组织酶抽提液的蛋白含量。但添加鱼王精或黑曲霉和康氏木霉处理的甜菜渣，会引起蛋白酶的活力降低；添加甜菜渣或经不同方法处理的甜菜渣均会引起淀粉酶活力明显升高或显著升高，对纤维素酶，则只有添加黑曲霉和康氏木霉处理的甜菜渣时，才会明显升高。 |
| 关键词: 中国对虾 消化器官 蛋白酶 淀粉酶 纤维素酶 甜菜渣 |
| DOI： |
| 分类号: |
| 基金项目:国家“九五”攻关专题资助项目，850240123号 |
附件 |
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| STUDIES ON THE EFFECTS OF BEET DREGS ON THE ACTIVITIES OF DIGESTIVE EGZYMS OF THE SHRIMP PENAEUS CHINENSIS |
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Luo Rixiang
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Institute of Oceanology , Chinese Academy of Sciences, Qingdao 266071
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| Abstract: |
| Shrimps(Penaeus chinensis) averaging 9.02cm in body length as the experimental material were cultured in our laboratory from June to September, 1996. The protein content of hepatopancreas, stomach and midgut were measured by Biuret reaction. The protease activity was measured by Folin-Phen reaction; the amylase and cellulose activity by the Somogyi method The shrimps were fed with dry supplemented added with 10% beet dregs or 10% various mould fermented beet dregs; and the effects of these two kinds of feed activities were studied. The results were as follows:
1. The protein content in hepatopancreas, stomach and midgut was 7.95 - 9.30 μg, and was not affected by the diet supplemented with 10% beet dregs or 10% beet dregs fermented by Trichoderma koningii and Aspergillus niger.
2. The protease activity in hepatopancreas, stomach and midgut was 0.892 μg/min in the shrimp fed with the diet supplemented with 10% beet dregs; and 0.222 μg/min for the shrimps fed with 10% beet dregs fermented by T. koningii and A. niger.
3. The amylase activities were increased in the shrimps fed the diet supplemented with 10% beet dregs or 10% beet dregs fermented by T. koningii and A. niger and 3.349 μg/min, 3.711 μg/min respectively, and the control was 2.66 μg/min respectively.
4. The cellulase activity of the shrimps was increased only by the diet supplemented with 10% beet dregs fermented by T. koningii and A. niger, and was 0.451 μg/min. The cellulase activity of the shrimps fed with diet supplemented with 10% beet dregs was 0.1106 μg/min, and was 0.2043 μg/min in the control. |
| Key words: Penaeus chinensis, Digestive organs, Protease, Amylase, Cellulase, Beet dreg |
