摘要: |
1993年11月20日自鹿儿岛养殖业者购得自然产卵获得的牙鲆受精卵,经空运、陆运至福冈的九州大学附属水产试验所。仔鱼翌日孵出,3d后开口。以此材料观察研究试验室条件下不同海水盐度对牙鲆仔稚鱼的生长、存活率及白化率的影响。试验分4组,盐度分别为:1组(自然海水对照组),30.5-31.8;2组,26.0;3组,21.0;4组,16.0。每组设2个重复。使用1001透明玻璃钢水槽,每槽放2000尾刚开口的仔鱼。仔鱼投喂轮虫、卤虫幼体,试验进行51d。结果表明,各不同盐度海水中仔稚鱼的生长无显著差别(P>0.05)。各组的存活率为:1组(对照组),26.9%及27.3%;2组,23.8%及35.6%;3组,41.5%及40.2%;4组,62.9%及42.1%,各组的白化率为:1组,83.6%及81.5%;2组,76.6%及75.8%;3组,41.9%及33.8%;4组,34.8%及37.6%。较低盐度海水中仔稚鱼呈现较高的存活率和较低的白化率。盐度在21.0与26.0间,可能是一个对牙鲆仔稚鱼的存活率及白化率产生显著不同结果的临界盐度阶段。 |
关键词: 盐度 牙鲆 仔稚鱼 生长 存活率 白化率 |
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EFFECTS OF SALINITY ON GROWTH, SURVIVAL RATE AND ALBINISM RATE OF THE LARVAE AND JUVENILES OF PARALICHTHYS OLIVACEUS |
Wang Hansheng
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Fujian Fishery Research Institute, Xianmen 361012
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Abstract: |
Seawater salinity's effects on growth, survival rate and albinism rate of larvae and juveniles of Paralichthys olivaceus were studied under laboratory condition. Fertilized eggs (spawned on Nov. 19th l993) were purchased and transported to the Fishery Research Laboratory of Kyushu University. The larvae hatched on Nov. 20th and opened their mouths 3 days later.
Four different levels of salinity were set as follows: control (30.5 - 3l.8), 26.0, 21.0, 16.0. Duplicate tanks were established for each salinity level. Eight 1001 transparent polycarbonate circular tanks inside a vinyloid hothouse were used to culture 2000 3-day-old larvae in each of them. The tanks’ seawater temperature was 17.8 - 21.8°C; light density was controlled at 800—1000 h by curtains. Airstones, and flowing water were applied during culture of the larvae, fed on rotifers and Arremia naupli. The volumes of the air current, flowing water, and food, were increased appropriately in accordance with the growth of the larvae. The experiment period lasted 51 days (from Nov. 21th 1993 to Jan. 11th 1994). During the experiment period, 30 individuals were sampled every 7 days (every 5 days from Dec. 22th) to measure the total length of the fishes by profile projector after anaesthesia with MS-222. When the fishes completed metamorphosis and became benthonic (mean total length 18 mm), all were taken out, anaesthetized with MS-222 and preserved in 10% neutral formalin to calculate the numbers of the individuals of normal and abnormal in body color and compare the growth, survival rate and albinism rate. Survival rate (%) = N2/(N0-N1)×100 (N2 = No. of surviving ind.; N0 = Beginning No.; N1 = Sampled No.). The color types were distinguished as follows: Type 1: normal; Type 2: trunk normal, with head albinotic partly; Type 3: trunk normal, with head albinotic; Type 4: head normal, with trunk albinotic partly; Type 5: both head and trunk albinotic partly; Type 6: head all albinotic, with trunk albinotic partly; Type 7: head normal, with trunk all or almost albinotic; Type 8: head partly albinotic, with trunk all or almost albinotic; Type 9: both head and trunk all or almost albinotic.
The results showed that the mean total length of the fishes of each experiment tank was 18.7 - 20.2mm, no distinguished differences in statistics available (P> 0.05). The survival rates of the experiment fishes were as follows: Group 1:26.9% and 27.3%; Group 2: 23.8% and 35.6%; Group 3: 41.2% and 40.2%; Group 4: 62.9% and 42.1%. The albinism rates were: Group 1: 83.6% and 81.5%; Group 2: 76.6% and 75.8%; Group 3: 41.9% and 33.8%; Group 4: 34.8% and 37.6%. The types of albinism could be basically classified into three types: type 4:, type 5 and type 9. Other types of albinism did not existent or were not apparent.
The study showed that from salinify of 16.0 to 31.8: 1. different level of salinity had no notable effects on the growth of the larvae and juveniles of Paralichthys olivaceus; 2. the lower the salinity the higher the survival rate and the lower the albinism rate of the larvae and juveniles of the fish; 3. salinity from 26.0 to 21.0 may be the critical range causing notable differences in the survival rate and albinism rate of the juveniles of the fish. |
Key words: Salinity, Paralichthys olivaceus, Larva juvenile, Growth survival rate, Albinism rate |