| 摘要: |
| 于1996年10月在青岛胶州湾附近海域采集中国对虾、细巧仿对虾、周氏新对虾、鹰爪虾、脊尾白虾和脊腹褐虾等6种不同科或不同属的海产虾类，用RAPD技术对其基因组DNA的多态性进行了研究。在事先优化的反应条件下，经20个随机引物扩增，共得到282条多态性片段，片段长度在230-2800bp之间。根据扩增片段的共享度计算出相对遗传距离指数，然后用UPGMA和NJ程序进行聚类分析，结果所显示的6种虾的亲缘关系与传统的分类结果基本一致，说明RAPD在海洋动物的遗传学研究中是一种有重要价值的遗传标记。 |
| 关键词: RAPD 海产虾类 基因组DNA 遗传距离 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目，3947014号;国家攀登计划B资助项目，PD-B6-5-3号;中国科学院院长基金资助项目，963054号 |
附件 |
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| STUDIES OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS ON GENOMIC DNA POLYMORPHISM IN SIX SPECIES OF MARINE SHRIMP |
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SONG Lin-sheng, XIANG Jian-hai, ZHOU Ling-hua, ZHANG Shou-lin, LIU Rui-yu
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Institute of Oceanology,the Chinese Academy of Sciences,Qingdao,266071
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| Abstract: |
| Random amplified polymorphic DNA (RAPD) technique was applied to study the polymorphism of genomic DNA of six marine shrimps in different families or genera Penaeus chinensis, Parapenaeopsis tenella, Metapenaus joyneri, Trachypenaeus curvirostris, Exopalaemon carinicauda and Grangon affinis, which were collected from Jiaozhou Bay, Qingdao in Oct. 1996. Amplifications with 20 primers under predetermined optimal reaction conditions (samples were first heated at 94°C for 5 min. and followed by 45 cycles of 1 min at 94°C. 1 min at 36°C, and 2 min at 72°C, then held at 72°C for 10 min) gave 282 reproducible amplified fragments ranging between 230 and 2800bp. The amplified fragments were scored as present (1) or absent (0) for each DNA sample and an index of degree of band sharing (F) was calculated by using Nei and Li’s matching coefficient method. The value of (1–F) was used to evaluate genetic distances between species. The phylogenetic trees were constructed with the method of bootstrap on the basis of the presence and absence of the amplified polymorphic DNA fragments, and with the methods of UPGMA and NJ on the basis of genetic distances. The results from the three methods of cluster analysis are similar in general. And the relationships indicated by the phylogenetic trees show the difference between orders, families, genus and species. The results are in good overall agreement with classical taxonomy. It is suggested that random amplified polymorphic DNA (RAPD) approaches are as useful in providing markers for marine animal genetic as they have been for other species. |
| Key words: RAPD, Marine shrimp, Genomic DNA, Genetic distance |
