| 摘要: |
| 于1996年5月和10月在黑龙江省肇东市东发渔场两次对1995年5月用静水压休克法人工诱导并在池塘养殖的三倍体鲶鱼以及对照组二倍体鲶鱼随机取样进行性腺组织解剖和显微观察。结果表明，雄性三倍体鲶鱼的精巢发育与对照组二倍体鲶鱼基本保持在相同的发育水平上，均能完成从精原细胞、初级精母细胞经减数分裂形成精子细胞的过程，但是，三倍体鲶鱼精巢的壶腹腔中精子细胞和成熟精子都明显少于二倍体鲶鱼，而且尚能见到少量畸型精子。雌性三倍体鲶鱼的卵巢处于未分化的卵原细胞阶段，而二倍体鲶鱼卵巢发育正常，已达到卵黄泡出现时相（第III时相）。此外，雌性二、三倍体鲶鱼的卵巢重量明显不同，GSI（性腺成熟系数）差异显著。 |
| 关键词: 三倍体鲶鱼 精巢 卵巢 |
| DOI：10.11693/hyhz200002002002 |
| 分类号: |
| 基金项目:“八五”农业部重点生物技术项目，生85－02－04－02号 |
附件 |
|
| STUDY ON GONADAL DEVELOPWENT OF TRIPLOID CATFISH (SILIRUS ASOTUS L.) |
|
YIN Hong-bin1, SUN Zhong-wu2, PAN Wei-zhi1, CHEN Hai-yan1
|
|
1.Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070;2.College of Wildlife Resource, Northeast Forestry University, Harbin, 150040
|
| Abstract: |
| Triploid catfish (Silurus asotus L.) were induced by hydrostatic pressure shock and raised in fishpond in Dongfa Fishery in Zhaodong City of Heilongjing Province in May, 1995. In May, 1996, 18 catfish including 9 triploid and 9 diploid and in October, 1996, 26 catfish (including 13 triploid and 13 diploid) were sampled randomly. The gonad of the sample triploid catfish was dissected and observed histologocally through microscope and compared with that of the control diploid catfish, the fish ploidy was determined by chromosome analysis and erythrocyte nuclear measurement. After the fish was dissected, the gonad was taken out and weighed and immobilized in Bouin solution before a series of alcohol dehydration, then embedded in paraffin, cut into 4–5μm sections, dyed with H.E dye and sealed with neutral resin, and finally observed through Olympass microscope and photomicrographed.
The results of the gonadal observation indicate that the testis development of male triploid catfish could keep up with that of diploid catfish, and meiotic course, i. e. development from spermatogonia, primary spermatocytes to spermatoblasts could be finished in maletriploid catfish. However, developing sperms and mature sperms in the cavity of testis of triploid catfish were obviously less than those in diploid catfish, and a small amount of heteromorphic sperms and sterile sperms could be detected. Whether the triploid catfish could reproduce after attaining complete sexual maturation remained a question to be clarified.
The ovary development of female triploid catfish was entirely different from that of diploid catfish: for all the sample female triploid catfish, the ovary was at the undifferentiated oogonium stage, while the ovary of the control diploid catfish developed normally and reached phase III. Therefore, female triploid catfish may be sterile.
Moreover, during the course of dissecting and observing, the ovary of female triploid catfish was excessively small due to underdevelopment; its weight only accounted for 0.08%, of its body weight, while the ratio for female diploid catfish was up to 1.43%, nearly 20 times of the former, so the ploidy of female catfish can be determined directly and accurately by GSI, as has been proved by chromosome analysis and erythrocyte nuclear measurement. However no marked difference of GSI between male diploid catfish and male triploid catfish was observed. |
| Key words: Triploid catfish (Silurus asotus L.), Ttetis, Ovary |
