引用本文:	刘晓萍,王玉贞,韩彦弢,于业军,王跃军,王春波.扇贝多肽在体外对免疫细胞活性的影响及其抗紫外线的氧化损伤作用.海洋与湖沼,2001,32(4):414-419.

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扇贝多肽在体外对免疫细胞活性的影响及其抗紫外线的氧化损伤作用
刘晓萍¹, 王玉贞¹, 韩彦弢¹, 于业军¹, 王跃军², 王春波¹
1.青岛大学医学院青岛266021;2.中国水产科学研究院黄海水产研究所青岛266071

摘要:

采用噻唑盐(MTT)比色方法研究在紫外线辐射损伤条件下扇贝多肽对免疫细胞的保护作用,并探讨扇贝多肽对胸腺细胞和脾细胞活性的影响。结果表明,扇贝多肽具有抗紫外线氧化损伤的作用,可减轻或抑制紫外线对胸腺细胞和脾细胞的氧化损伤,并且呈剂量依赖性。扇贝多肽在0.5%—10.0%的浓度范围内,其抗氧化能力随浓度的增高而增强;在正常条件下可显著增强免疫细胞的活性,并且可拮抗雌激素对免疫细胞的抑制作用。提示扇贝多肽不仅具有抗氧化损伤作用,而且具有免疫增强作用。

关键词: 扇贝多肽免疫细胞紫外线损伤

DOI：

分类号:

基金项目:国家自然科学基金资助项目39970638号

附件

EFFECT OF POLYPEPTIDES FROM CHLAMYS FARRERI ON THE PROLIFERATION OF IMMUNE CELLS AND ITS PROTECTION AGAINST ULTRAVIOLET IN VITRO

LIU Xiao-Ping¹, WANG Yu-Zhen¹, HAN Yan-Tao¹, YU Ye-Jun¹, WANG Yue-Jun², WANG Chun-Bo¹

1.Medical College of Qingdao University,Qingdao,266021;2.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,266071

Abstract:

The polypeptides isolated from Chlamys farreri (PCF) by modern bioengineering technique are a water soluble small molecular with biological activity. In this paper the effects of PCF on the proliferative activities of thymocytes and splenocytes and the protective effects of PCF on those immune cells under Ultraviolet (UV) oxidative damage were detected by MTT colorometry. The results showed that PCF could reduce the ultraviolet (UV) damage on the thymocytes and splenocytes with dose dependence. In the 0.5%–10.0% concentration range the higher concentration of PCF the stronger protective effects of it. PCF could also significantly increase the proliferative activities of the thymocytes and splenocytes and could decrease or inhibit the immune suppressive effects of Estradiol on the thymocytes and splenocytes. The results indicate that PCF not only has the effect of antioxidative damage of UV irradiation but also has the effect of increasing immune function.

Key words: Polypeptides from Chlamys farreri (PCF), Immune cells, Ultraviolet damage