| 摘要: |
| 于1998年4、5月在山东省荣成市寻山水产公司养鱼场人工采集牙鲆成熟精卵,紫外线灭活精子遗传物质,冷休克诱导,获得雌核发育牙鲆。1999年5月收集57尾雌核发育牙鲆生化样品,采用淀粉胶电泳分析了LDH、IDHP、PGM、ACP、ADH、SDH、GDH、CAT等8种在野生种群中为多态的同工酶。结果表明,牙鲆雌核发育群体的L dh-C、Cat两个基因座位发生了重组,基因座位与着丝点之间的重组率分别为52.6%和29.8%;I dhp-1有父方基因表达现象,说明雌核发育过程中父方基因可能在分子水平整合;牙鲆雌核发育群体的多态座位比例和平均杂合度分别为7.0%和0.035,远低于野生种群;研究表明,通过抑制第二极体排出获得的牙鲆雌核发育子代尚有一定基因杂合,不能用于培育纯系。
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| 关键词: 牙鲆,雌核发育,同工酶,基因重组,父方基因表达 |
| DOI：10.11693/hyhz200201010010 |
| 分类号: |
| 基金项目:国家“863”计划资助课题,963052号 |
附件 |
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| RECOMBINATION AND EXPRESSION OF PATERNAL GENE OF ISOZYMES IN GYNOGENETIC OLIVE FLOUNDER PARALICHTHYS OLIVACEUS |
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XU Cheng, WANG Ke-Ling, XU Yong-Li, ZHANG Pei-Jun
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Institute of Oceanology,The Chinese Academy of Sciences
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| Abstract: |
| Gynogenetic induction of olive flounder Paralichthys olivaceus was carried out by cold shock after activation of the eggs with sperm which had been inactivated with UV irradiation. Biochemical samples of 57 gynogenetic flounder (in a family) were collected in May, 1999. 8 isozymes, including LDH, IDHP, PGM, ACP, ADH, SDH, GDH, CAT, were analyzed by starch gel electrophoresis. These isozymes were polymorphic in wild population. Two loci, L dh-C and Cat had recombination in gynogenetic population. The recombination rates between isozyme locus and centromere were 52.6% and 29.8%, respectively. Paternal gene of I dhp-1 was found expressing at a low level in gynogenetic progenies. Mean proportion of polymorphic loci and the average heterozygosities of gynogenetic population were 7.0% and 0.035, respectively, much lower than that of wild population. The result suggests that gynogenesis involving retention of the second polar body is not a good method for making pure lines, and gynogenesis involving suppression of the first cell division may be feasible.
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| Key words: Paralichthys olivaceus, Gynogenesis, Isozyme, Recombination, Expression of paternal gene |
