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| 引用本文: | 孙虎山,李光友.硒化卡拉胶和酵母葡聚糖对栉孔扇贝血淋巴中两种水解酶活力的影响.海洋与湖沼,2002,33(3):245-249. |
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| 硒化卡拉胶和酵母葡聚糖对栉孔扇贝血淋巴中两种水解酶活力的影响 |
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孙虎山1, 李光友2
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1.烟台师范学院生物科学与技术系;2.国家海洋局海洋生物活性物质重点实验室
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| 摘要: |
| 对栉孔扇贝分别注射硒化卡拉胶和酵母葡聚糖后, 采用生化方法, 于1h、15h 和30h分别测定栉孔扇贝血清和血细胞中2 种参与免疫防御的水解酶酸性磷酸酶( ACP) 和溶菌酶的活力。结果表明, 注射硒化卡拉胶后, 栉孔扇贝血清和血细胞中ACP 活力在1h 和30h 时均为实验组显著高于对照组, 溶菌酶活力也均在1 和30h 时实验组显著高于对照组。注射酵母葡聚糖后, ACP 活力在1h、15h 和30h 时均为实验组极显著地高于对照组, 溶菌酶活力则均为实验组与对照组差异不显著。说明硒化卡拉胶对栉孔扇贝血淋巴中ACP 和溶菌酶的活力均有增强作用, 而酵母葡聚糖仅对其ACP 活力有增强作用。 |
| 关键词: 硒化卡拉胶, 酵母葡聚糖, 栉孔扇贝, 血淋巴, 水解酶 |
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| 基金项目:国家"973"课题资助项目, G1999012005 号 |
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| THE EFFECTS OF SELENO-CARRAGENNAN AND β-1, 3-GLUCAN ON THE ACTIVITIES OF HYDROLYTIC ENZYMES IN THE HAEMOL YMPH OF CHLAMYS FARRERI |
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SUN Hu-Shan1, LI Guang-You2
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1.Biological Science and Technology Department, Yantai Normal College;2.Key Lab of Marine Bioactive Substances, State Oceanic Administration
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| Abstract: |
| Two kinds of hydrolytic enzymes to participate in the immune defense in the haemolymph of Chlamys farreri were assayed at 1h, 15h and 30h after injection with seleno-carragennan or β-1, 3-glucan. After injection with seleno-carragennan, the acid phosphatase (ACP) activities of experimental groups in serum and haemocytes were much higher than that of control groups at 1 and 30h; the differences of lysozymal activities of experimental and control groups in serum and haemocytes were significant except the group in serum after 15h. After injection with β-1, 3-glucan, the ACP activities of experimental groups in serum and haemocytes were much higher than that of control groups at 1h, 15h and 30h; the differences of lysozymal activities of experimental and control groups both in serum and haemocytes were not significant at 1h, 15h and 30h. It was suggested that the Seleno-carragennan could enhance the activities of ACP and lysozyme in serum and haemocytes of Chlamys farreri, but the β-1, 3-glucan could only enhance the activities of ACP. |
| Key words: Seleno-carragennan, β-1, 3-glucan, Chlamys farreri, Haemolymph, Hydrolytic enzyme |
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