引用本文:	叶海辉,李少菁,李祺福,黄辉洋,王桂忠.生物胺对雌性锯缘青蟹生殖神经内分泌的调控作用.海洋与湖沼,2003,34(3):329-333.

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生物胺对雌性锯缘青蟹生殖神经内分泌的调控作用
叶海辉^1,2, 李少菁², 李祺福¹, 黄辉洋², 王桂忠²
1.厦门大学生命科学学院细胞生物学研究室;2.厦门大学海洋系

摘要:

采用活体注射和体外培养方法, 进行生物胺调控雌性锯缘青蟹生殖神经内分泌的研究。结果表明, 锯缘青蟹于实验的第1天, 第5天, 第10天, 分别注射100μl剂量为1μg/g体重的5羟色胺(5-HT)、章鱼胺(OA)和多巴胺(DA),第15天解剖；其中5-HT对卵巢发育具有显著的促进作用, OA和DA的作用不明显。离体条件下, 将脑、胸神经团、视神经节分别与卵巢小块进行联合培养, 每个实验组分别加入PBS与1μg/g体重的5-HT、OA、DA、HA(组胺)和NE (去甲肾上腺素), 发现卵巢发育的促进作用与5-HT刺激了脑和胸神经团的分泌活动有关。

关键词: 生物胺,锯缘青蟹,卵巢发育,神经内分泌

DOI：10.11693/hyhz200303012012

分类号:

基金项目:福建省重中之重“福建省海洋生物优良种质和生物活性物质的应用基础研究”项目资助1998—2002年;高等学校博士学科点专项科研基金项目资助,20010384010号

附件

THE EFFECTS OF BIOGENIC AMINES ON OVARIAN DEVELOPMENT IN THE MUD CRAB, SCYLLA SERRATA

YE Hai-Hui^1,2, LI Shao-Jing², LI Qi-Fu¹, HUANG Hui-Yang², WANG Gui-Zhong²

1.Laboratory of Cell Biology, School of Life Science, Xiamen University;2.Department of Oceanography, Xiamen University

Abstract:

The possibility that biogenic amines affect ovarian development in the female mud crab, Scylla serrata, was investigated. Females were administered 1μg/g body weight of 5-hydroxytryptamine (5-HT), octopamine (OA) or dopamine (DA) on days 1, 5 and 10 and were sacrificed on day 15. Crabs given 5-HT showed significant increases in ovarian index and oocyte size over the concurrent controls, while OA and DA did not significantly affect either the ovarian index or oocyte size. In vitro study indicates that none of 5-HT, OA, DA, histamine (HA) and norepinephrine (NE) has direct stimulation over the ovarian development. 5-HT stimulates the ovarian development, presumably by triggering release of gonad-stimulating hormone in the brain and the thoracic ganglion, while the other 4 amines have no evident effects upon the brain, the thoracic ganglion and optic ganglion.

Key words: Biogenic amine, Scylla serrata, Ovarian development, Neurosecretion