引用本文:	李太武,杨文新,宋林生,苏秀榕,杨志彪,郭　皓.皱纹盘鲍(Haliotis discus hannai Ino)和杂色鲍(Haliotis diversicolor Reeve)遗传多样性的RAPD研究.海洋与湖沼,2003,34(4):444-449.

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*皱纹盘鲍(Haliotis discus hannai* Ino)和杂色鲍(Haliotis diversicolor Reeve)遗传多样性的RAPD研究**
李太武¹, 杨文新², 宋林生³, 苏秀榕¹, 杨志彪¹, 郭　皓⁴
1.宁波大学生命科学与生物工程学院;2.辽宁师范大学生命科学学院;3.中国科学院海洋研究所;4.国家海洋环境监测中心

摘要:

于2001年8月—2002年7月, 选用20条能产生清晰可重复扩增产物的随机引物, 对皱纹盘鲍(Haliotis discus hannai Ino)、杂色鲍(Haliotis diversicolor Reeve)进行了RAPD分析, 共检测到213个位点, 在群体之间和个体之间都存在差异。利用PopGen32软件计算各群体扩增位点的多态性比例、群体遗传杂和度和群体间的遗传距离, 多态比例分别为43.66%、53.05%, 2个群体的平均杂合度为0.1557、0.1686。结果表明皱纹盘鲍与杂色鲍的亲缘关系较远。

关键词: 鲍,RAPD,遗传多样性

DOI：10.11693/hyhz200304012012

分类号:

基金项目:浙江省自然科学基金资助项目,400002号

附件

EXPLORING ON THE GENETIC POLYMORPHISM IN HALIOTIS DISCUS HANNAI INO AND H. DIVERSICOLOR REEVE BY RAPD TECHNIQUE

LI Tai-Wu¹, YANG Wen-Xin², SONG Lin-Sheng³, SU Xiu-Rong¹, YANG Zhi-Biao¹, GUO Hao⁴

1.Life Science and Technology School, Ningbo University;2.Life Science College, Liaoning Normal University;3.Institute of Oceanology, The Chinese Academy of Sciences;4.National Marine Environment Monitoring Center

Abstract:

Twenty random primers screened out of forty ones were used to detect the polymorphism of Haliotis discus hannai Ino and Haliotis diversicolaor Reeve. The total of 213 clear and informative amplified fragments was obtained. There was diversity not only among the two populations but also between the individuals. The population of Haliotis diversicolaor Reeve had several specific fragments different from H. discus hannai. The percentage of polymorphic loci genetic identity and genetic distance were calculated to reveal the genetic variations among populations by means of PopGen32 software, the percentages of polymorphic loci of Haliotis diversicolaor Reeve, Haliotis discus hannai Ino are 53.05%, 43.66%, and the average genetic heterozygosities are 0.1686, 0.1557, respectively. The result indicated that there was a relatively distant relationship between Haliotis diversicolar Reeve and H. discus hunnai.

Key words: Abalone, RAPD, Genetic polymorphism