| 引用本文: | 赵元凤,吕景才,吴益春,宋晓阳,王凡,刘长发,赵冲,郑伟.铜在牙鲆(Paralichthys olivaceus)组织蓄积、分配及排放的研究.海洋与湖沼,2005,36(2):139-145. |
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| 铜在牙鲆(Paralichthys olivaceus)组织蓄积、分配及排放的研究 |
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赵元凤1, 吕景才1, 吴益春2, 宋晓阳2, 王凡2, 刘长发1, 赵冲2, 郑伟2
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1.农业部海洋水产增养殖学与生物技术重点开放实验室 大连116023,大连水产学院生命科学与技术学院 大连116023;2.大连水产学院生命科学与技术学院 大连116023
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| 摘要: |
| 采用暴露实验方法, 研究了海水中Cu在牙鲆(Paralichthys olivaceus)内脏、肌肉、鳃组织内吸收、积累和排放规律, 海水中总有机碳(TOC)浓度、配体种类对铜吸收的影响及其与海水表观络合容量(ACuCC)的关系。结果表明, Cu浓度为0.5mg/L时, 各组织内Cu蓄积量随暴露时间增加而增大, 第13天均达吸收平衡, 此时Cu蓄积量(mg/kg)为内脏971.89>肌肉204.99>鳃90.04。染毒13天牙鲆在清洁海水中排放结果表明: 随排放时间增加各组织Cu蓄积量下降, 第8天接近排放平衡。此时各组织Cu排出率为: 肌肉89%>鳃86.5%>内脏团83.7%。海水中TOC浓度、种类对牙鲆Cu蓄积有明显影响, 当TOC浓度相同时, 孔石莼分泌物比牙鲆分泌物更能降低Cu在牙鲆组织内吸收和蓄积量; 当TOC种类相同时, 随TOC浓度升高, 各组织铜蓄积量均明显下降, 表明海水TOC能降低牙鲆组织对Cu的吸收和积累。ACuCC随TOC浓度增加有明显上升趋势, 并与TOC浓度呈线性相关。 |
| 关键词: 铜 牙鲆 蓄积 总有机碳(TOC) |
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| 基金项目:国家自然科学基金资助项目 ,30271029号 |
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| ACCUMULATION, DISTRIBUTION AND EXCRETION OF MARINE COPPER CONTAMINATION IN THE TISSUES OF PARALICHTHYS OLIVACEUS |
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ZHAO Yuan-Feng1,2, Lü Jing-Cai1,2, WU Yi-Chun2, SONG Xiao-Yang2, WANG Fan2, LIU Chang-Fa1,2, ZHAO Chong2, ZHENG Wei2
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1.Key Laboratory of Mariculture and Biotechnology, Ministry of Agriculture, Dalian, 116023;2.College of Life Science and Technology, Dalian Fisheries University, Dalian, 116023
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| Abstract: |
| The authors studied the behavior of marine contaminant copper in biological insights of the accumulation, distribution and excret ion in or from visceral mass, muscles and gills of Paralichthys olivaceus. Effects of total organic carbon (TOC) and its concentration in seawater on the accumulation of copper in Paralichthys olivaceus, and the relationship between TOC and the apparent complex capability of seawater to copper (ACUCC) were studied. It was demonstrated that when copper concentration is 0. 5mg/L, the copper content in every party of the tissue increase with time. In the 13th day, copper accumulation in the tissues reached equilibrium with the figures of 971.89mg/kg in visceral mass, >204.99mg/kg in muscles and > 90.04mg/kg in gills. During purification days, the copper concentration in the animal body decreased with time. In the 8th day of the cleaning, the equilibrium reached. The percentages of Cu excretion were 89% in muscle, > 86.5% in gill and > 83.7% in visceral mass. Concentration of TOC in the seawater had significant effect on copper accumulation. It was found that at the same TOC concentration, Ulva pertusa secretion could reduce the copper accumulation better than that of Paralichthys olivaceus. In the same species, the copper accumulation decreased significantly with increasing TOC. This is because that TOC in seawater can reduce the bioavailability of copper. On the other hand, the ACUCC has linear positive relat ion with the TOC.
In this experiment, sample P. olivaceus are in average (4.77±0.98)cm long and (4.13±0.12)g weight. They were raised for 5 days before the experiment for the purpose of totally expelling the faeces. To avoid the effect of diets and faeces on the distribution of heavy metals, the fish were not fed in the period of experiment.
Experiment on copper accumulation in Paralichthys olivaceus: 5 days after being raised, every 50 Paralichthys olivaceus are placed indoor in two 120L glass jars with 80L Cu-poisoned seawater solution; the Cu concentration was 0.5mg/L. Recharge the solution daily with the toxicant to keep the original concentration. At the same frequency, 3 animal individuals are sampled. Sampled fish are dissected in to part of gills, visceral mass and muscle. Each part is dehydrated, weighted, dissolved and neutralized; the copper accumulation is then measured on Metrohm 746 VA Trace Analyzer. When equilibrium of copper accumulation in the fish body is reached, transfer the remaining fish to two the same jars in 80L pure seawater. Refresh daily with the pure seawater daily. Sample is taken every two days. The copper accumulation and samples are measured in the same manner. The experiment lasted 13 days.
The measurement of ACUCC and KC: 20ml water sample are placed in 6 clean and dried small beakers by pipet, then the copper standard liquid are placed in each beakers by microsyringe to make the concentrations of copper to be 0, 10, 20, 30, 40, 50Lg/L. 24 hours later, copper and ligand were fully complexed, and then free copper ions in each beaker were measured. A relational formula was established based on the 1:1 ratio of Cu complexation and organic ligands. ACUCC and KC of pure seawater and seawaters with different TOC could be measured according to the above method. |
| Key words: Copper, Paralichthys olivaceus, Accumulation, Total organic carbon (TOC) |
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