| 摘要: |
| 以荧光分光光度检测法和小麦胚芽鞘切段生物试法对16种中国东部沿海生长的海洋藻类中的生长素含量进行了定量分析。结果表明，所测定的16种海藻，包括红、绿及褐藻中均有生长素(IAA)存在。海藻不同种类之间的含量变化较大，褐藻中的含量低于红藻和绿藻。小麦胚芽鞘切段生物试法对IAA测定的含量变化为5.3–110.2ng/gFW之间，荧光分光光度检测法测定的含量变化为1.1–46.9ng/gFW。小麦胚芽鞘切段生物试法对IAA测定的含量明显高于荧光分光光度检测法测定的相同种含量。讨论结果认为，小麦胚芽鞘切段生物试法的测定结果虽然灵敏度高，但易受实验材料不均一性的制约，试验周期长，并受环境因子的干扰，适用于海藻中生长素的半定量分析；荧光分光光度检测法相对适合于海藻中生长素的定量分析，有很好的可行性。 |
| 关键词: 海藻 植物生长素 荧光分光光度检测法 小麦胚芽鞘切段生物试法 含量分布 |
| DOI： |
| 分类号: |
| 基金项目:国家863高技术研究发展计划资助项目,2001AA620503和2004AA625030号;中国科学院方向性创新项目 ,KZCX3-SW-215号 |
附件 |
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| ISOLATION AND ESTIMATION OF AUXIN FROM MARINE ALGAES |
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HAN Li-Jun, FAN Xiao, YUAN Zhao-Hui
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Institute of Oceanology,Chinese Academy of Sciences,Qingdao,266071
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| Abstract: |
| Auxin are important phytohormones. Many researches have showed that they are involved intensively in plant growth, development, and many other activities and functions. However, the study on auxin in marine algae in China was unfortunately rare. The main reason for not being able to study was the tiny amount of auxin in marine algae.
The present research shows that auxin exists in more than one forms in plant tissues, but the most important compound might be indole-3-acetic acid (IAA). IAA activity in seaweed extract was also found by Standerson in 1987 and confirmed that IAA in seaweed extract could increase crop yield, improve plant growth, and tolerate frost, fungal and insect attack.
Research in this paper included methods of IAA measurement, existence of IAA and its concentration in Chinese marine algae. The seaweed extract with IAA is promising to be used in agriculture as plant promoter.
According to Leopold (1955), a series of process including pigment reduction with petroleum benzine were used to extract and isolate auxin from marine algae. Wheat coleoptile by biological detection and indole-α-pyrone fluorescence methods were carried out to estimate the content and distribution of auxin in 16 Chinese seaweeds. The differences between the two methods were also discussed. The results shows that auxin are widely distributed in marine algae. In this experiment, auxin were detected in every alga sample, and the concentrations varied with species. The results measured by wheat coleoptile by biological detection and indole-α-pyrone fluorescence methods showed the same distribution pattern. Results also shows that indole-α-pyrone fluorescence method is better than the method of biological detection. The concentration of auxin in phaeophyta is lower than those in Phodophyta and Chlorophyta. |
| Key words: Marine algae, Indole-α-pyrone, Fluorescence method, Wheat coleoptile by biological detect method, Content distribution |
