| 摘要: |
| 采用静态毒性实验方法,研究重金属镉胁迫条件下大弹涂鱼外周血微核率和肝脏过氧化物酶活性的变化。结果表明,在0.05mg/L Cd浓度组,10d时检测到微核率极显著升高;0.5mg/L和5mg/L组5d时就可检测到微核率极显著升高,到10d时微核率达到最高值;10d时转入清洁海水后,3个组在15d再次出现一个微核率的最高值。大弹涂鱼肝脏过氧化物酶标记包括谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽转硫酶(GST)。3个组的GSH-Px酶活性和5mg/L浓度组GST酶活性在12h后显示出极显著变化,3个组GST酶活性在24h检测到极显著差异;在转入清洁海水5d时,3个组GSH-Px酶活性和对照组相比可检测到极显著差异,而3个组的GST酶活性均无显著差异。研究结果表明,大弹涂鱼外周血微核标记和肝脏过氧化物酶标记能够灵敏地指示水环境中的镉污染;大弹涂鱼外周血微核标记和肝脏内过氧化物酶标记具有一定的互补作用。
关键词 镉, 大弹涂鱼, 微核, 谷胱甘肽过氧化物酶, 谷胱甘肽转硫酶 |
| 关键词: 镉,大弹涂鱼,微核,谷胱甘肽过氧化物酶,谷胱甘肽转硫酶 |
| DOI： |
| 分类号: |
| 基金项目:浙江省科技厅资助项目，2005C32020号 |
附件 |
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| CADMIUM-INDUCED CHANGES IN THE MARKERS OF ERYTHROCYTE MINCRONULEI AND PEROXYDASES IN LIVER OF BOLEOPHTHALMUS PECTINIROSTRIS |
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Zhang Chun-dan, Huang Fu-yong, Li Ming-yun, Lu Sun-jie, Zhu Jun-yong, Wu Yong
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Faculty of Life Science and Biotechnology, Ningbo University, Ningbo
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| Abstract: |
| The potential utility of erythrocyte micronuclei (MN) and peroxydases in liver as indicators of exposure to cadmium chloride with the toxic effects in marine fish Boleophthalmus pectinirostris were evaluated. Three concentration groups (0.05mg/L, 0.5mg/L and 5mg/L) of Cd2+ and a control group were enacted, and each group repeats it for another two times. The erythrocyte MN of B. pectinirostris in the first group (0.05mg/L) of Cd2+ increased significantly up to 10d while the erythrocyte MN of B. pectinirostris in the latter two groups increased significantly in the fifth day and reached to maximum up to 10d and then declined. The fish of three concentration groups were moved into clean seawater after 10-day exposure, and another maximum of the MN frequency would appear 15 days later. The peroxidases in the liver of B. pectinirostris, including the glutathione peroxidases (GSH-Px) and glutathione S-transferases (GST), were more sensitive than the marker of erythrocyte MN when the water area w as polluted by the heavy metal of cadmium. GSH-Px activity of the three concentration groups would display extremely significant change compared with controls when the fish were exposed to the toxicant for 12h, and the change can be examined even the fish had recovered for 5 days in clean water. GST activity showed extreme significantly increase compared with control groups none but the 5mg/L groups. All the three concentration groups can be detected for significant decrease at 24h, and when the fish had been recovered for 5 days, all the three groups show no significant difference compared with the controls. The results show that the markers of erythrocyte micronuclei and peroxydase in liver of marine fish B. pectinirostris can denote the cadmium pollution sensitively in seawater. Its immunological memory responses bring out the maximum of MN frequency after cadmium removed. The diversification curve of the marker of peroxydases in liver is correlated tightly with the mechanism of the two peroxydases in eliminating free radicals and toxicants. B. pectinirostris may be a valuable biomaker in water pollution examination, and how to integrate the markers of erythrocyte micronuclei and peroxydase in liver to shape a new way to monitor marine environment is an interesting field, for the two markers each has its pros and cons. |
| Key words: Cadmium, Boleophthalmus pectinirostris, Micronuclei, The glutathione peroxidases, The glutathione S-transferases |
