引用本文:	杨志彪,赵云龙,周忠良,李娜,杨健.水体铜对中华绒螯蟹(Eriocheir sinensis)代谢酶活力的影响.海洋与湖沼,2006,37(2):118-124.

【打印本页】【HTML】【下载PDF全文】【查看/发表评论】【下载PDF阅读器】【关闭】

过刊浏览高级检索

本文已被：浏览 1601次下载 0次	码上扫一扫！
分享到：微信更多字体:加大+\|默认\|缩小-
*水体铜对中华绒螯蟹(Eriocheir sinensis)代谢酶活力的影响*
杨志彪¹, 赵云龙¹, 周忠良¹, 李娜¹, 杨健²
1.华东师范大学生命科学学院上海200062;2.中国水产科学研究院淡水渔业研究中心无锡214081

摘要:

采用生态学单因子梯度试验方法,研究了水环境中添加0.01、0.10、1.00、5.00mg/L的铜(Cu²⁺)对中华绒螯蟹肝胰腺、血淋巴和鳃代谢酶活力的影响。结果表明,随着Cu²⁺浓度的增高,肝胰腺和血淋巴的铜锌超氧化物歧化酶(Cu-Zn SOD)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活力和血蓝蛋白均呈先增加后减少的变化趋势。鳃钠钾三磷酸腺苷酶(Na⁺,K⁺-ATPase)、钙镁三磷酸腺苷酶(Ca²⁺,Mg²⁺-ATPase)和谷丙转氨酶(GPT)活力均随着Cu²⁺浓度的增高而降低。随着Cu²⁺浓度的增高,肝胰腺髓性过氧化物酶(MPO)活力呈先减少后增加的变化趋势。水环境中添加0.01mg/L Cu²⁺就可对中华绒螯蟹代谢酶产生显著影响,并随着Cu²⁺浓度的增高,对中华绒螯蟹机体的影响逐渐加剧。表明代谢酶活力的变化可以灵敏地反映Cu²⁺的胁迫程度和毒性。

关键词: 中华绒螯蟹,肝胰腺,血淋巴,鳃,代谢酶,铜

DOI：

分类号:

基金项目:中国水产科学研究院首席科学家资助金项目,1-105011号

附件

EFFECTS OF WATER-BORNE COPPER ON ACTIVITIES OF METABOLISM ENZYMES IN ERIOCHEIR SINENSIS

YANG Zhi-Biao¹, ZHAO Yun-Long¹, ZHOU Zhong-Liang¹, LI Na¹, YANG Jian²

1.School of Life Science, East China Normal University, Shanghai, 200062;2.Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081

Abstract:

Effect of water-borne copper Cu²⁺ in different concentrations (0.00, 0.01, 0.10, 1.00, 5.00mg /L) on activities of several main metabolic enzymes in hepatopancreas, hemolymph and gill of Eriocheir sinensis were studied by Single Gradient Factor Experiments. The result showed early rise-up and later fall-down curve in Cu-Zn SOD activity in hepatopancreas, with increasing of Cu²⁺ level and the turn-over point of 26.018U/mg at 1.00mg /L Cu²⁺. The maximum activities of ACP (170.861U/mg) and AKP (267.238U/mg) were observed at 0.10mg/L Cu²⁺, while the minimum activities of ACP (123.879U/mg) and AKP (133.244U/mg) were at 5.00mg/L Cu²⁺. The activity of MPO was significantly different between the controls and treated groups when Cu²⁺ level rises. The lowest (1.605U/g) and highest value (8.743U/g) of the enzyme were at the water-borne of 0.01mg/L Cu²⁺ and 5.00mg/L Cu²⁺, respectively. Gill impairment by Na⁺, K⁺-ATPase and Ca²⁺, Mg²⁺-ATPase activities occurred when exposed to water-borne Cu²⁺, obtaining the highest and lowest value in the controls and 5.00mg/L Cu²⁺ treated group, respectively. In hemolymph the activity of Cu-Zn SOD increased in the beginning and then decreased when Cu²⁺ concentration increased, highest (229.047U/ml) activities at 1.00mg/L Cu²⁺ and lowest (199.539U/ml) at 5.00mg/L Cu²⁺. The highest activity of GPT (38.132U/L) was obtained in the controls, while the lowest one (0.024U/L) was observed at 5.00mg/L Cu²⁺ group. Increasing Cu²⁺ level was also associated with the activity of ACP, showing early increase at the peak of 3.529U/100ml and later decrease being at the trough of 2.171U/100ml corresponding to 0.10 and 5.00mg/L Cu²⁺, respectively. Similarly the activity of AKP peaked to 2.496U/100ml at 0.10mg/L Cu²⁺ and dipped to 1.642U/100ml at 1.00mg/L Cu²⁺. In addition, hemocyanin activity in the hemolymph exhibited a trend of early up and later down, 485.227U/L the maximum at 0.10mg/L Cu²⁺ and the minimum 89.200U/L at 5.00mg/L Cu²⁺. It should be emphasized that a significant impairment to metabolism enzymes can occur even at 0.01mg/L Cu²⁺, a very low level that is in the range of National Water Quality Standard for Fisheries (GB11607-89). The changes in the activities of enzyme can be used as a sensitive biomarker to Cu²⁺ toxicity for E. sinensis. More species-specified national water quality standards are suggested to be applied to different economic animals.

Key words: Eriocheir sinensis, Hepatopancreas, Hemolymph, Gill, Metabolism enzyme, Copper