| 引用本文: | 卢圳域,朱伟明,顾谦群,刘培培,刘茂新,郭健,管华诗.海洋微藻的体外抗肿瘤活性初筛.海洋与湖沼,2007,38(5):464-467. |
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| 海洋微藻的体外抗肿瘤活性初筛 |
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卢圳域1, 朱伟明1, 顾谦群1, 刘培培1, 刘茂新1, 郭健2, 管华诗1
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1.教育部海洋药物重点实验室 山东省海洋药物重点实验室 中国海洋大学医药学院 青岛266003;2.中国海洋大学水产学院 青岛266003
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| 摘要: |
| 采用小鼠乳腺癌细胞株tsFT210的细胞周期抑制、细胞凋亡诱导、细胞坏死以及对卤虫的毒性为活性指标,对48种海洋微藻的甲醇提取物的抗肿瘤活性进行了筛选。结果表明,以卤虫毒性为活性指标时,9种海洋微藻显示了不同程度的抗肿瘤活性,以tsFT210为筛选模型时,也有9种具有抗肿瘤活性,其中3种为明显的细胞凋亡诱导活性、2种为细胞坏死性活性、1种为G2/M期抑制活性,因此有必要进行开拓海洋微藻药用新资源的研究。 |
| 关键词: 海洋微藻 抗肿瘤 卤虫毒性 细胞周期抑制 细胞凋亡 |
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| 基金项目:国家高科技发展计划资助,2003AA624020号;中国海洋大学科研启动基金资助,2003—2005 |
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| ANTI-TUMOR ACTIVITY SCREENING OF METHANOL EXTRACTS OF MARINE MICROALGAE IN VITRO |
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LU Zhen-Yu1, ZHU Wei-Ming1, GU Qian-Qun1, LIU Pei-Pei1, LIU Mao-Xin1, GUO Jian2, GUAN Hua-Shi1
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1.Key Laboratory of Marine Drug of Chinese Ministry of Education, Key Laboratory of Marine Drug of Shandong Province, School of Medicine and Pharmay, Ocean University of China,Qingdao,266003;2.College of Fisheries, Ocean University of China,Qingdao, 266003
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| Abstract: |
| Marine microalgae act as an important member in marine biological system with photosynthetic ability as major producers of biomass and organic compounds in the oceans. Many microalgal metabolites have unique structures and are formed in biosynthetic routes quite different from known terrestrial metabolites'. These marine metabolites showed potential bioactivities in vitro assay, such as anti-tumor, antivirus and immunosuppressive activities. For example, malyngamide J, from marine cyanobacterium Lyngbya majuscula, has been selected for in vivo evaluation by the NCI (National Cancer Institute) because of its unusual cytotoxicity in 60 cell lines and its structural novelty. However, the study on small molecule bioactive metabolites of marine microalgae in China has been unfortunately rare. In order to search for structurally novel and biologically active compounds from rich marine microalgae, 48 strains of marine microalgae were screened for their anti-tumor activities using brine shrimp, Artemia salina Leach, and the mouse temperature-sensitive p34cdc2 mutant cell line tsFT2l 0. The methanol extract of the mycelium of 9 strains of tested microalgae, Synedra fragilaroides B132, Ruttnera spectabilis H55, Cricosphaera gayraliae H13, Cylindrotheca sp. B165, Cylindrotheca sp. B171, Cylindrotheca sp. B177, Isochrysis galbana H29, Skeletonema costatum B129 and Cylindrotheca sp. B156 showed biotoxicity against A. salina at concentration of 2 mg/ml, and their lethality was 100%, 100%, 92%, 86%, 85%, 64%, 54%, 50% and 42%, respectively. At 2 mg/ml in concentration, the methanol extract from the mycelium of 8 strains in tested microalgae exhibited cytotoxicity against tsFT2l0 cell, among which Synedra fragilaroides B132, Platychrysis sp. H38, Ruttnera spectabilis H55, and Pseudostichococcus monallantoides C18 showed apoptosis inducing activity, and Cylindrotheca sp. B177, Cricosphaera gayraliae H13, Anabaena inaequalis Y335 and Cylindrotheca sp. B165 showed necrosis activity, and Cricosphaera sp. H58 arrested the cell cycle at G2/M phase by a flow cytometry. These results revealed that the metabolites of marine microalgae consisted of anti-tumor compounds and the marine microalgae. As a new pharmaceutical resource, further research is needed. |
| Key words: Marine microalgae, Anti-tumor, Brine shrimp toxicity, Cell cycle inhibition, Apoptosis |
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