引用本文:	张永正,张海琪,邵庆均,何中央.翘嘴红鲌(Erythroculter ilishaeformis Bleeker)种群遗传多样性的RAPD分析.海洋与湖沼,2007,38(6):581-584.

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*翘嘴红鲌(Erythroculter ilishaeformis* Bleeker)种群遗传多样性的RAPD分析**
张永正¹, 张海琪², 邵庆均³, 何中央²
1.浙江海洋学院杭州311258;2.浙江省水产技术推广总站杭州310012;3.浙江大学动物科学学院杭州310007

摘要:

采用RAPD技术对翘嘴红鲌养殖群体进行了DNA多态性检测。共采用40个随机引物进行扩增,其中14个引物可获得清晰且重复性好的扩增图谱,共扩增出121个RAPD位点,具有多态现象的位点为40个,多态位点比例为33.06%。翘嘴红鲌群体的遗传相似系数为0.8878-0.9600,平均为0.9201,遗传变异度为0.0799。Shannon多样性指数为10.8824,Shannon多样性值为0.0899。研究表明,翘嘴红鲌目前的种质资源状况令人堪忧,改变目前人工繁育模式,建立原种、良种场,丰富物种遗传多样性是资源保护的基础。

关键词: 翘嘴红遗传多样性 RAPD

DOI：

分类号:

基金项目:浙江省财政重点资助项目“翘嘴红优质苗种繁育及健康高效养成技术示范与推广”,2004—2006;浙江省科技厅重大攻关项目,2004C12025号

附件

THE GENETIC DIVERSITY OF GERMPLASMS OF ERYTHROCULTER ILISHAEFORZVIS BLEEKER REVEALED BY RAPD MARKERS

ZHANG Yong-Zheng¹, ZHANG Hai-Qi², SHAO Qing-Jun³, HE Zhong-Yang²

1.Zhejiang Ocean University, Hangzhou, 311258;2.Zhejiang Fishery Technical Extension Center, Hangzhou, 310012;3.College of Animal Science, Zhejiang University, Hangzhou, 310007

Abstract:

Random amplified polymorphic DNA (RAPD) technique was employed to analyze the genetic diversity of cultivation stock of Erythroculter ilishaeformis Bleeker. Amplification was done with 40 random primers, of which 14 produced distinctive and reproducible fingerprints. 121 sites were detected, and 40 of them were polymorphic. Genetic similarity among E. ilishaeformis populations varied 0.8878 - 0.9600 in average of 0.9201; and the genetic variability was 0.0799. Shannon index of phenotype diversity was 10.8824 and the Shannon’s information index was 0.0899. The study indicates that the germplasm resource of the species was in a worrying situation, changing the current mode of artificial breeding, establishing more breed- and stock- farms, and enhancing the genetic diversity of the species are strongly recommended for protecting the fish resource.

Key words: Erythroculter ilishaeformis Bleeker, Genetic diversity, RAPD