| 摘要: |
| 以南极硅藻GJ01（Berkeleya rutilans）为对象,分别采用紫外、可见和荧光分光光度法,研究了营养条件等因子对GJ01生长和胞内谷胱甘肽含量的影响,并对培养的GJ01细胞合成谷胱甘肽的条件进行了优化。结果表明,在单因子影响条件下,1.2g/L的碳酸氢钠对南极硅藻GJ01的生长最好,且最有利于谷胱甘肽的积累;0.8mmol/L氯化钙对GJ01的生长最为有利,而0.2mmol/L氯化钙对谷胱甘肽的产生是适宜的;维生素B12和生物素对GJ01胞内谷胱甘肽的产生影响较小。正交试验表明,GJ01细胞产生谷胱甘肽的最优条件是:光照周期为16h/8h、NaHCO3为1.6g/L、Ca2+为0.4mmol/L、Cys为10mmol/L。该结果为利用南极硅藻GJ01生产谷胱甘肽提供了参考和依据。 |
| 关键词: 南极硅藻，谷胱甘肽，GJ01，培养法， 优化 |
| DOI：10.11693/hyhz200801005005 |
| 分类号: |
| 基金项目:国家自然科学基金，40206022号、40406003号 |
附件 |
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| THE OPTIMAL CONDITION FOR GSH PRODUCTION FROM ANTARCTIC ICE MICROALGAE BERKELEYA RUTILANS GJ01 |
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DING Yu1,2, MIAO Jin-Lai3, LI Guang-You3, JIAN Ji-Chang1,2, WU Zao-He1,2
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1.Fisheries College of Guangdong Ocean University;2.Guangdong Provincial Key Lab of Pathogenic Biology and Epidemiology for Aquatic Economic Animals;3.Key Lab of Marine Bioactive Substances,State Oceanic Administration
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| Abstract: |
| GSH, as a key component of Asc-glutathione cycle, plays important roles in protecting tissues from toxicant damage and keeping cells in normal redox state, on which this paper studied. Berkeleya rutilans GJ01, one of so-called Antarctic ice microalgae, an extreme microorganism in the crysphere, is one of major producers of primary production in marine ecology system of Antarctic polar region. They have become a promising resource of GSH. In this paper, effect of nutrition factor on GSH production level was studied for GJ01 with spectrophotometer in searching for the optimization of synthesis condition for GSH. It was showed that 1.2g/L NaHCO3 and 0.2mmol/L Ca2+ is ideal to the production of GSH. Vitamin B12 and biotin impacted slightly on GSH production. Orthogonal test revealed that the optimal condition for GSH production of GJ01 is 16h light/8th dark, 1.6g/L NaHCO3, 0.4mmol/L Ca2+, and 10mmol/L Cys. The results should be informative to the application of GJ01 for GSH production. |
| Key words: Berkeleya rutilans, Glutathione, GJ01, Cultural method, Optimization |
