摘要: |
采用生物毒性测试与评价方法对常用消毒剂药物三氯异氰尿酸(Trichloroisocyanuric acid,TCCA)对剑尾鱼的急性毒性及其Ⅰ、Ⅱ相代谢酶活性影响进行了研究。结果表明,TCCA对剑尾鱼的96h LC50为2.15mg/L。TCCA对剑尾鱼肝脏还原型谷光苷肽(GSH)、谷胱苷肽硫转酶(GST)和7-乙氧基异吩恶唑酮-脱乙基酶(EROD)都存在诱导作用。雌雄个体在GST和EROD的诱导响应时间有显著差异性,其中雄性个体GST对TCCA暴露响应比雌性个体敏感。 |
关键词: 三氯异氰尿酸 剑尾鱼 毒性效应 抗氧化酶 |
DOI:10.11693/hyhz200805010 |
分类号: |
基金项目:国家自然科学基金资助项目,40471118号、U0633006号;科技部公益研究专项资助项目,2004DIB1Z-029号 |
附件 |
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TOXICITY AND ITS EFFECTS UPON THE ANTIOXIDANT ENZYMES OF TRICHLOROISOCYANURIC ACID TO SWORDTAIL FISH XIPHOPHORUS HELLERI |
NIE Xiang-Ping1, WANG Xiang1,2, LI Kai-Bin3, WU Shu-Qin3
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1.Institute of Hydrobiology, Jinan University, Guangzhou, 510632;2.Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan, 430079;3.Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, 510380
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Abstract: |
The toxicity and effect of TCCA (trichloroisocyanuric acid) upon swordtail fish Xiphophorus helleri were studied in acute toxicity tests with the values of biomarkers in Phase I and Phase II . The biomarkers included activities of EROD (7-ethoxyresorufin-O-deethylase), GST (glutathione S-transferase) and total GSH (glutathione). Results showed that TCCA was highly noxious to the swordtail fish during early exposure period. The 96h LC50 of TCCA was 2.15 mg/L. Induction effects were observed for GSH, EROD and GST in liver tissues of swordtail fish under the exposure of TCCA. Among them, GST and EROD were more sensitive to the exposure of TCCA and may be used as alternative biomarkers of TCCA exposure. However, some differences were noticed between female and male individuals for GST and EROD in response time to TCCA exposure; male fish were more sensitive to TCCA. In term of EROD, female individuals showed persistent induction during the whole exposure period, while male ones did so only in the early and middle period of the exposure and decrease afterwards. |
Key words: Trichloroisocyanuric acid, Swordtail fish Xiphophorus helleri, Toxicity effect, Antioxidant enzyme |